基于D-loop和Cyt b序列的大海马遗传变异分析 |
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引用本文: | 袁吉贵,罗杰,刘丽,张艳苹,梁万宁.基于D-loop和Cyt b序列的大海马遗传变异分析[J].安徽农业科学,2017,45(36):140-142,235. |
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作者姓名: | 袁吉贵 罗杰 刘丽 张艳苹 梁万宁 |
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作者单位: | 广东海洋大学水产学院,广东湛江,524088;湛江溢鑫源水产科技有限公司,广东湛江,524088 |
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摘 要: | 目的]对大海马D-loop和Cyt b序列进行遗传变异分析。方法]基于D-loop和Cyt b部分序列对东山岛(20尾)和泉州市(20尾)养殖场大海马进行遗传变异分析。结果]获得的D-loop和Cyt b序列分别为707~709 bp和404 bp,且2种序列在2个大海马群体之间极其保守。D-loop和Cyt b序列G+C含量明显低于A+T含量,C含量也低于G含量,Cyt b序列第3位密码子G含量最少。东山岛和泉州市的大海马(各20条)D-loop序列的平均核苷酸差异数分别为0.489和0.958,且分别定义了3种和6种单倍型,东山岛和泉州市的大海马(各20条)Cyt b序列在2个群体之间相似度为100%,只定义了1种单倍型。结论]研究表明D-loop和Cyt b序列在2个大海马群体中变异较小。
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关 键 词: | 遗传变异 大海马 D-loop Cytb |
Analysis of Genetic Variation of Hippocampus kuda Base on D-loop and Cyt b Sequences |
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Abstract: | Objective] To study the genetic variation of Hippocampus kuda base on D-loop and Cyt b sequences. Method] In this paper, the genetic variations of H.kuda between Dongshan island(20 individuals) and Quanzhou City(20 individuals) were analyzed base on part of D-loop and Cyt b sequences. Result] D-loop and Cyt b sequences were 707 -709 bp and 404 bp, respectively, and the two sequences were highly conserved between the two populations.G+C content of D-loop and Cyt b sequences were significantly lower than A+T, however, the C content was lower than G, and G content of Cyt b sequences were the least in the third codon.The average number of nucleotide differences of 20 D-loop sequences of H.kuda from Dongshan island and Quanzhou City were 0.489 and 0.958, and 3 and 6 kinds of haplotypes were defined respectively, meanwhile, the similarity of Cyt b sequences were 100% with a total of 1 haplotype between the two groups. Conclusion] All the results showed that the variation of D-loop and Cyt b sequences were relatively little in the two population of H.kuda. |
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Keywords: | Genetic variation Hippocampus kuda D-loop Cyt b |
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