超声提取优选研究多叶棘豆各药用部位盐酸小檗碱的分布

目的]比较2种超声提取工艺研究多叶棘豆茎、叶、根、种子中盐酸小檗碱分布的不同。方法]采用2种超声提取工艺对蒙药多叶棘豆茎、叶、根、种子中盐酸小檗碱进行提取并优选(方法Ⅰ是先将蒙药进行超声提取后放置24 h,方法Ⅱ是将蒙药先室温放置24 h再进行超声提取)。在波长426 nm处用分光光度法测定,计算盐酸小檗碱的含量和回收率。结果]多叶棘豆茎中盐酸小檗碱分布最多,叶、根、种子盐酸小檗碱分布明显减少,而2种提取工艺中方法Ⅱ优于方法Ⅰ,加样回收率在92.58%~110.00%,RSD在0.782 3%~0.974 9%。结论]多叶棘豆各药用部位盐酸小檗碱分布不均,茎中分布明显多,2种不同超声提取工艺中方法Ⅱ优于方法Ⅰ。

Study on the Distribution of Berberine Hydrochloride in Various Medicinal Parts of Oxytropis myriophy by Ultrasonic Extraction Op-timization

Objective] The research aimed to compare the distribution of berberine hydrochloride in the stem, leaf, root and seed of Oxytropis myriophy by two different ultrasonic extraction techniques. Method] Two kinds of ultrasonic extraction process were used to extract and optimize the berberine hydrochloride in the stems, leaves, roots and seeds of Oxytropis myriophy(The first technology was to extract the Mongolian medicine first by ultrasonic extraction and then placed 24 h, and the second technology was to put the Mongolian medicine at room temperature for 24 h before ultrasonic extraction.).And calculated the content of berberine hydrochloride and recovery in 426 nm wavelength spectrophotometry.Result]The content of berberine hydrochloride was the most in the stalk of Oxytropis myriophy, the distribution of berberine hydrochloride in leaves, roots and seeds was obviously reduced,and the second technology of extraction process was better than the first technology , the recovery rate was 92.58％-110.00％, the relative standard deviation (RSD) was 0.7823％-0.9749％.Conclusion]The distribution of berberine hydrochloride is not equal in the medicinal parts of Oxytropis myriophy, and the distribution in the stems is much more significantly, and the second technology is better than the first in the two different ultrasonic extraction techniques .