Effects of digestible protein levels in isonitrogenous diets on growth performance and tissue composition of juvenile Atractoscion nobilis

A study was undertaken to estimate the effects of isonitrogenous diets (ca. 604 g kg^?1 crude protein) containing formaldehyde-treated (FT) fish meal and graded levels of digestible protein (DP) (541, 491, 372, 347 and 247 g kg^?1) on growth performance and tissue composition of juveniles white seabass. Five diets were formulated to contain increasing levels of FT fish meal (from 0 to 384 g kg^?1) and decreasing levels of non-treated fish meal (from 480 to 96 g kg^?1). Each dietary treatment was fed in triplicate to apparent satiation to groups of 25 fish for 50 days. Significantly higher growth performance and feed conversion ratio were obtained in fish-fed diets containing 491 or 541 g kg^?1 DP, compared with all other treatments. Apparent digestibility coefficient of protein in the diets was not significantly affected by the inclusion of treated fish meal in the diets. Estimation of protein requirements using a broken-line regression analysis indicated that maximum weight gain would be obtained with a diet containing 503 ± 23 g kg^?1 DP. The results from this study suggest that a single-diet formulation using protein treated with formaldehyde as filler might be useful to estimate the requirement of DP for fish.