HPLC和UV法对比测定蚊不叮霜中VB1的含量

【目的】 对比研究高效液相色谱法(HPLC)和紫外可见分光光度法(UV)测定蚊不叮霜中维生素B₁含量。为高效、快速、准确测定蚊不叮霜中维生素B₁的含量奠定基础。【方法】 UV法的检测波长为246 nm,采用百分吸收系数法计算维生素B₁的含量;HPLC法检测波长为267 nm,色谱柱选用C18柱。【结果】 UV法在7.47～14.94 μg/mL范围内线性关系良好(r=0.999 3),精密度实验RSD=0.30%,重复性实验RSD=1.04%,平均回收率105.13%,RSD=1.72%,样品在处理后30 min内稳定(RSD=0.91%);HPLC法在4.85～11.31 μg/mL范围内线性关系良好(r=0.9999),精密度实验RSD=0.28%,重复性实验RSD=0.90%,平均回收率101.77%,RSD=1.93%,样品在处理后12 h内稳定(RSD=0.39%)。【结论】 HPLC法测得三批样品的平均含量分别为8.39、8.39、8.45 mg/g;UV法测得三批样品的平均含量分别为8.73、8.70、8.77 mg/g。UV法及HPLC法的精密度、重复性均较好,但UV法测得含量及回收率均偏高,测定准确度不如HPLC法,且UV法稳定性较差,故最终确定采用HPLC法测定蚊不叮霜中维生素B₁的含量。

Comparative Determination of Vitamin B1 in Mosquitoes Repellent Cream by HPLC and UV

【Objective】 The content of vitamin B₁ in mosquito repellent cream comparatively determine by HPLC and UV spectrophotometry. 【Methods】 The detection wavelength of UV spectrophotometry 246 nm, and the content of vitamin B₁ calculated by the method of percentile absorption coefficient; the detection wavelength of HPLC 267 nm, chromatographic column C18. 【Results】 The results showed that the UV method good linear relationship in the 7.47~14.94 μg/ml range (r=0.999,3), RSD of precision experiment 0.30%, RSD of repetitive experiment 1.04%, the average recovery rate was 105.13%, RSD1.72%, and the sample was stabilized within 30 minutes after treatment (RSD=0.91%); the HPLC method good linear relationship in the 4.85~11.31 μg/ml range (r=0.999,9), RSD of precision experiment 0.28%, RSD of repetitive experiment 0.90%, the average recovery rate was 101.77%, RSD1.93%, and the sample was stabilized within 12 hours after treatment (RSD=0.39%). 【Conclusion】 The average contents of three batches of samples determined by HPLC method were 8.39, 8.39 and 8.45 mg/g, respectively. The average contents of the three batches of samples were 8.73, 8.70 and 8.77 mg/g. The precision and repeatability of UV and HPLC method both good, but the content and recovery of UV method were higher than of labeled amount, the accuracy of determination of UV was not as good as that of HPLC method. Furthmore, the stability of UV method was poor. Therefore, HPLC method more suitable for determining the content of vitamin B₁ in mosquito repellent cream.