盐环境下氮素对苗期盐角草氮同化关键酶活性的影响

【目的】 研究盐环境下施氮水平对盐角草幼苗对氮催化和同化的能力,了解盐角草对氮素吸收转化的机制。【方法】 采用盆栽试验方法,研究不同施氮水平对盐环境下生长的盐角草幼苗氮同化关键酶活性的影响。【结果】 与不施氮处理相比,在重度盐环境条件下,施氮可现在提高盐角草幼苗根、茎、同化枝NR、GS、GOGAT、GLDH的活性;施N(1.2 g/kg)时,盐角草幼苗根、茎、同化枝活体NR活性、茎活体GS、GOGAT活性达到最大值;施N(2.4 g/kg)时,盐角草幼苗根、同化枝活体GS、GOGAT活性及根、茎、同化枝活体GLDH活性达到最大值。【结论】 盐环境下施氮能够显著增强盐角草幼苗氮同化关键酶活性,提高了盐角草对氮素胁迫能力,增强其对高氮环境的适应性。

Effects of Nitrogen on the Activities of Key Enzymes for Nitrogen Assimilation of Salicornia europaea L. during Seedling Stage

【Objective】 In order to reveal the nitrogen catalysis and assimilation capacity of Salicornia europaea L. seedlings under nitrogen application in salt environment, and to deeply understand the mechanism of the grass to nitrogen uptake and transformation. 【Method】 The pot experiment method was used to study the effects of different nitrogen application levels on the key enzyme activities of nitrogen assimilation of Salicornia europaea L. seedlings growing under salt environment. 【Results】 The results showed that, compared with the treatment without nitrogen application, under severe salt environment conditions, nitrogen application increased the activities of roots, stems, assimilated shoots NR, GS, GOGAT, GLDH of the plant. Under N 1.2 g/kg, the NR activity, GS, GOGAT activity of the root, stem, and assimilated branches of Salicornia europaea L. seedlings reached their maximum values; When N (2.4 g / kg) was applied, the roots and assimilated branches of GS, GOGAT activity and GLDH activity of root, stem and assimilated branches reached the maximum. 【Conclusion】 Nitrogen application in salt environment can significantly enhance the key enzyme activities of nitrogen assimilation of Salicornia europaea L. seedlings, increase its ability to nitrogen stress, thus enhancing its adaptability to high nitrogen environment.