野生型根癌农杆菌对马铃薯的遗传转化研究

用不同的野生型根癌农杆菌(Agrobacterium tumefaciens)T37、B6、TFA16YET、FA16YK对马铃薯普通栽培种甘农薯1号的块茎圆片、茎段、叶切片、试管苗,美国引进品种褐皮.布尔班克的块茎圆片以及新型栽培种NW-174实生苗的子叶及下胚轴等组织进行了离体侵染和整株侵染实验,发现:不同的菌种对同一组织以及相同的菌种对不同组织的侵染效果均不同。在供试菌株中,以TFA16YE的转化效果最好。对NW-174新型栽培种实生苗的子叶、下胚轴进行的侵染实验表明,这两种组织的转化率较高。结果表明,在离体转化程序中,外植体的生理状态、发育阶段以及供试菌株的代谢活性及培养条件等均是影响转化效率的重要因素。

Study on the Genetic Transformation of Potato with Wild Type Agrobacterium tumefaciens

In the studies,tuber discs,stem segments,leaf sections and the test tube plantlets from Solanum tuberosum cv.Gannongshu No.1.Russet burbank and the cotyledons and hypocotyls from a Neo-tuberosum breeding line NW-174 were infected in vitro with Agrobacterium tumefaciens strains T37,B6,TFA16YE and TFAl6YK.It was found that the infection effects were different among the bacterium strains in the same infected tissue as well as different among the different tissues infected with the same bacterium strain.The bacteria TFA16YE had a best transformation effect.The cotyledons and hypocotyls from the NW-174 had the highest transformation efficiency.The results showed that the physiological and developmental status of explants,the metabolism characters and culture conditions of the bacteria were the critical factors affecting genetic transformation efficiency.