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| 山羊（Bore）-兔异种克隆胚胎连续核移植的研究 | |
| 引用本文： | 章志国,章孝荣,刘亚,金仁桃,章美玲,汪存利,赵焕. 山羊（Bore）-兔异种克隆胚胎连续核移植的研究[J]. 中国农业科学, 2005, 38(3): 601-605 |
| 作者姓名： | 章志国 章孝荣 刘亚 金仁桃 章美玲 汪存利 赵焕 |
| 作者单位： | 1. 安徽农业大学动物科技学院,合肥,230036;安徽医科大学第一附属医院妇产科医学生殖中心,合肥,230022 2. 安徽农业大学动物科技学院,合肥,230036 3. 安徽省应医院,合肥,230022 |
| 基金项目： | 安徽省“十五”重大科技专项资助项目(01603006) |
| 摘 要： | 以山羊-兔异种克隆桑椹胚卵裂球为核供体，兔卵母细胞为受体进行连续核移植研究，最终连续2次，共获得继Ⅰ代重构胚58枚，继Ⅱ代重构胚14枚。融合率分别为79.5%和70%，差异不显著（P>0.05）卵裂率分别为75.9%和28.6%,差异显著（P<0.05）。但继Ⅱ代重构胚最终未能发育至囊胚，继Ⅰ代重构胚囊胚率达到10.2%。 |
| 关 键 词： | 山羊 兔 异种克隆 连续核移植 |
| 收稿时间： | 2003-12-25 |
Study of Serial Nuclear Transfer on Goat (Bore)-Rabbit Inter-Species Cloned Embryo |
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| ZHANG Zhi-guo,ZhANG Xiao-rong,LIU Ya,JING Ren-tao,ZHANG Mei-ling,WANG Cun-li,ZHAO Huan. Study of Serial Nuclear Transfer on Goat (Bore)-Rabbit Inter-Species Cloned Embryo[J]. Scientia Agricultura Sinica, 2005, 38(3): 601-605 | |
| Authors: | ZHANG Zhi-guo ZhANG Xiao-rong LIU Ya JING Ren-tao ZHANG Mei-ling WANG Cun-li ZHAO Huan |
| Affiliation: | ZHANG Zhi-guo1,2,ZhANG Xiao-rong1,LIU Ya1,JING Ren-tao3,ZHANG Mei-ling1,WANG Cun-li1,ZHAO Huan1 |
| Abstract: | The experiments of serial nuclear transfer were conducted by using the blastomere of inter-species original or serial cloned morula as donor and rabbit oocyte as recipient. Finally serial I and serial II cloned embryos were obtained. Of these, Ⅰserial reconstructed embryos 58 andⅡserial reconstructed embryos 14 .The rate of fusion was 79.5% and 70% respectively and there was no significant difference between them(P>0.05). However, the rate of cleavage respectively was 75.9% and 28.6% and difference between them was very significant (P<0.05). Eventually there was noⅡserial blastocyst obtained, but the rate of Ⅰserial blastocyst was 10.2%. |
| Keywords: | Goat Rabbit Inter-species clone Serial nuclear transfer |
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