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In vitro plantlet regeneration of Olea europaea ssp. maderensis
Affiliation:1. National Researches Institute of Rural Engineering, Water and Forests, Street Hédi El Karray El Menzeh IV, BP N° 10, Ariana 2080 Tunisia;2. Faculty of Mathematical, Physical and Natural Sciences of Tunis, University of El Manar 2092, Tunisia;1. Research and Innovation Centre, Genomics and Biology of Fruit Crops Department, Fondazione E. Mach di San Michele all’Adige, 380101 San Michele all’Adige (TN), Italy;2. Department of Life Sciences and Systems Biology, Innovation Centre, University of Turin, Via Quarello 15/A, 10135 Turin, Italy;1. State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, China;2. School of Natural Resources, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China;1. Leibniz Universität Hannover, Institute of Horticultural Production Systems, Woody Plant and Propagation Physiology Section, Herrenhäuser Str. 2, 30419 Hannover, Germany;2. Institut für Pflanzenkultur e. K., Solkau 2, 29465 Schnega, Germany;1. Irrigation and Crop Ecophysiology Group, Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS, CSIC), Avenida Reina Mercedes, no. 10, Sevilla 41012, Spain;2. Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain;1. College of Life Sciences, Northeast Agricultural University, Harbin 150000, Heilongjiang, PR China;2. College of Life Sciences, Jiamusi University, Jiamusi 154007, Heilongjiang, PR China;3. College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China
Abstract:Different media were assayed for Olea europaea L. ssp. maderensis Lowe micropropagation. Shoot elongation and propagation was more efficient on DKW medium supplemented with 4.4 μM BA and 0.4 μM IBA. Higher branching was obtained on OM medium supplemented with 18.2 μM zeatin. Rooting was achieved at higher rates on half strength DKW medium supplemented with 20.7 μM IBA. Plants were acclimated to greenhouse and up to now no morphological changes were observed among the regenerated in vitro plants.
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