A Wheat Xylanase Inhibitor Protein (XIP-I) Accumulates in the Grain and has Homologues in Other Cereals |
| |
| Affiliation: | 1. Área de Microbiología, Postgrado de Edafología, Colegio de Postgraduados, Carretera México-Texcoco, km 36.5, Montecillo, 56230, Estado de México, México;2. Departamento de Biología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, Iztapalapa 09340, Distrito Federal, Mexico;3. Laboratorio Divisional de Microscopia Confocal, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, Iztapalapa 09340, Distrito Federal, Mexico;1. Department of Pharmacy Practice and Administration, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey;2. Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey;3. NJ Bariatric Center, Springfield, New Jersey;4. Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey;5. Pharmacokinetic/Pharmacodynamic Shared Resource, Rutgers Cancer Institute of New Jersey, New Brunswick, New Jersey;6. Department of Statistics and Biostatistics, Rutgers, The State University of New Jersey, Piscataway, New Jersey;7. ID Care, Hillsborough and Robert Wood Johnson University Hospital Somerset, Somerville, New Jersey;3. Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2781-901 Oeiras, Portugal;4. Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, Portugal;1. Departamento de Biología Celular y Molecular, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu, 9, 28040 Madrid, Spain;2. Section of Microbiology, Imperial College London, Flowers Building, Armstrong Road, London SW7 2AZ, UK;3. Department of Genetics and Evolution, University of Adelaide, Adelaide, South Australia 5005, Australia;4. Dept. of Applied Chemistry, Faculty of Chemistry, University of the Basque Country, Manuel de Lardizabal, 3, 20018 San Sebastian, Spain |
| |
| Abstract: | We have measured xylanase inhibitor activity against an Aspergillus niger xylanase in different parts of the wheat plant at different stages of development and used immunodetection to determine the spatial and temporal distribution of xylanase inhibitor protein I (XIP-I) in Triticum aestivum var. Soisson. Although xylanase inhibitor activity was detected in all parts of the wheat plant throughout development, XIP-I was located predominantly in the grain tissue, where it appears at a late stage in grain development and persists after germination, indicating that the different xylanase inhibitor proteins are under different regulatory controls. (1,4)-β-Xylanase activity was detected in wheat grains during development and post-germination. Pure XIP-I and a crude sample containing TAXI inhibitors but not XIP-I did not have the ability to inhibit this endogenous (1,4)-β-xylanase activity. Protein extracts from the seeds of various monocots were also tested for the presence of XIP-I, where it was found to be present in the grains of several wheat varieties, rye and barley, but was not detected in rice, sorghum or maize. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
