Identification by suppression subtractive hybridization and expression analysis of Arabidopsis thaliana putative defence genes during Orobanche ramosa infection |
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| Affiliation: | 1. Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland;2. Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland;1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling 712100, Shaanxi, China;2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100091, China;3. Hybrid Rapeseed Research Centre of Shaanxi Province, Yangling 712100, Shaanxi, China;1. School of Agriculture, Polytechnic Institute of Bragança, Campus Sta Apolónia Apt 1172, 5301-855 Bragança, Portugal;2. CECAV, Animal and Veterinary Research Centre, Portugal;3. CIMO, Mountain Research Centre, Portugal;4. University of Trás-os-Montes and Alto Douro, Quinta de Prados, Apt 1013, 5001-801 Vila Real, Portugal;1. VetAgro Sup, 89 Avenue de L''Europe, 63370, Lempdes, France;2. Université Clermont Auvergne, INRA, UMRF, F-15000, Aurillac, France;3. Université D''Artois, EA 7394, Institut Charles VIOLLETTE, Lens, F-62300, France;4. UMR Ingénierie Procédés Aliments, AgroParisTech, INRA, Université Paris-Saclay, 91300, Massy, France |
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| Abstract: | A suppression subtractive hybridization strategy was used to identify genes that were induced 2 h after infection of Arabidopsis thaliana by broomrape (Orobanche ramosa) seedlings. Among these genes, the expression of twelve was analysed from the first hour to the seventh day of this compatible interaction by cDNA blot analyses. The twelve genes showed a transient expression, which occurred in seven cases as early as the first or second hour of interaction and ended 2 or 3 h later. Most of the proteins encoded by these genes are already known to be involved in different A. thaliana response pathways to pathogen attack. The first two genes to be induced (Rc kinase and ACaM5) encoded a receptor and a calmodulin, and could be involved in signal transduction. The two following genes encoding a sucrose carrier (SUC1) and a protein with a pectin methylesterase inhibitor domain were found to be overexpressed; their roles are consistent with plant defence emergence. The gst1, gst11 and peptide methionine sulfoxide reductase genes, which were turned on early, are known to play a role in the detoxification of reactive oxygen species. The accumulation of mRNAs for lox1, a latex allergen and a myrosinase binding protein could be related to a jasmonate dependent pathway, while genes for a class III peroxidase and a caffeoyl-CoA 3-O-methyltransferase, both likely to be involved in cell wall reinforcement, were also upregulated. |
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