In vitro spore germination of the fern Schizaea dichotoma |
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| Affiliation: | 1. State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730010, PR China;2. Grasslands Research Center, Palmerston North, New Zealand;3. Altay Gaubau Tea Co., Ltd., Altay 836500, PR China |
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| Abstract: | Spores of Schizaea dichotoma were disinfested using sodium hypochlorite (NaOCl), streptomycin, or a combination of both streptomycin and NaOCl. Only the spores treated with streptomycin germinated. The highest germination (34%) was recorded in MS1/4 C compared to MS1/2, BB, or MM medium. The spore germination was not affected by the presence or absence of light or mycorrhizal fungi (Glomus clarum). The germinated spores developed up to five cells and failed to develop into a mature gametophyte. |
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