含玉米pepc基因恢复系的MAS转育及其杂交稻的光合特性和杂种优势研究

用玉米pepc基因的特异性引物对转育pepc基因的后代材料进行分子标记辅助选育（MAS），成功地培育出与轮回亲本蜀恢881遗传相似度达97.09%~99.03%的改良蜀恢881，用此改良恢复系与3个不育系冈46A、776A、2480A形成组合。结果表明,（1）自行设计的pepc基因特异性引物能对基因进行准确的筛选，而且玉

Photosynthetic Characteristics and Heterosis in Transgenic Hybrid Rice with Maize Phosphoenolpyruvate Carboxylase (pepc) Gene by MAS

Posterity materials carrying maize pepc gene has been successfully applied into marker-assisted selection(MAS)by using specific primer of maize pepc gene,and the improved Shuhui 881 with pepc gene has been obtained.The improved Shuhui 881 was similar to its backcross parents in genetic background with 97.09%-99.03%.Then rice sterile lines Gang 46A,776A,2480A as the female parents were crossed with several improved Shuhui 881 above.Some progress on photosynthetic characteristics and agronomic traits in hybrid rices with pepc gene were achieved.Firstly,Maize pepc gene not only could been correctly screened by MAS using specific primer designed by BLAST,but also was stably inherited and had PEPCase activity highly expressed in different genetic backgrounds.Secondly,PEPCase activity increased significantly as compared with respective controls and there was a significant correlation(0.6081)between PEPCase activity and Pn in hybrid rice with pepc gene.Thirdly,the photosynthetic indexes of transgenic hybrid rice such as apparent quantum yield(AQY),carboxylation efficiency(CE),were obviously superior to respective controls.Moreover,the performances in agronomic traits varied among hybrid rice with pepc gene except in 103-grain weight and grain yield per plant.Transgenic hybrid rice increased by average of 41.48% in grain yield per plant as compared with controls.And finally,according to the effect analysis of general combining ability(GCA),the 776A,Line 1 and Line 6 had outstanding performances,which was worth of further study in the future.