早期断奶对沂蒙黑山羊羔羊生长性能、盲肠短链脂肪酸含量和菌群多样性的影响

为分析早期断奶对沂蒙黑山羊羔羊生长性能、盲肠短链脂肪酸含量和菌群多样性影响,选用0d沂蒙黑山羊羔羊36只,分为2组,对照组和试验组,每组18只羔羊.母乳组(B组)羔羊哺喂母乳,代乳粉组(R组)于8 d起断奶,10 d完全哺喂代乳粉,15 d开始两组均饲喂开食料.在8、10、15、25、45和75 d时每组随机屠宰3头羊...

Effects of early weaning on growth performance cecum flora and short chain fatty acid content of Yimeng black lamb

The purpose of this study was to investigate the effects of early weaning on thegrowth performance, cecal short-chain fatty acid content and bacterial diversity of Yimeng black lamb. A total of 36 0-day Yimeng black lambs were selected and divided into two groups, control group and experimental group, with 18 lambs in each group. The lambs in breast milk group(group B)were fed with breast milk, while lambs in the milk replacer group(group R)were weaned and fed milk replacer from 8 days of age. Both of groups were fed with appetizer from 15 days of age. Three lambs in each group were randomly slaughtered at 8, 10, 15, 25, 45 and 75 days of age and theircecal contents were collected to analyze the content of short-chain fatty acids by GC-MS, and thebacterial diversity was investigated by high-throughput sequencing and their correlation. The results showed as follows: 1)The body weight of lambs in group R was significantly lower than that in group B at 15 days(P<0. 01), and was significantly higher than that in group B(P<0. 05)at 75 days. The daily gain of lambs in group R was significantly lower than that in group B at 11-15 days(P<0. 01), and was significantly higher than that in group B after 25 days(P<0. 05). 2)The contents of acetic acid, propionic acid, butyric acid, valeric acid and isovaleric acid at 25 d in group R were significantly higher than those at 15 d(P<0. 05), while the contents of all kinds of short chain fatty acids at 45 d in group B were significantly higher than those at 25 d(P<0. 05). 3)Sobs, Ace, Chao1 at 15 days of age and Shannon and Simpson at 25 days of age in group R were significantly lower than those in group B(P<0. 05). At the phylum level, the relative abundance of Firmicutes in group R was significantly lower than that in group B on day 25(P<0. 05). The relative abundance of Proteobacteria phylum was significantly higher than that in group B on days 10 and 45(P<0. 01). The relative abundance of Bacteroidetes phylum was significantly lower than that of group B on 75 days(P<0. 05). At the genus level, the relative abundance of Butyric-Ioccus was significantly decreased in both groups from day 10(P<0. 05), and extremely significantly decreased on day 25(P<0. 01). The relative abundance of Lactobacillus in group R was significantly lower than that in group B on day 15(P<0. 05). Peptoclostridium, Clostridium_Sensu_Stricto_1, Romboutsia, Intestinibacter, Terrisporobacter and Lachnospiraceae_nk3a20 group from Firmicutes are closely related to daily weight gain and production of short-chain fatty acids at 25 d. In conclusion, early weaning can reduce the daily gain and cecal microflora diversity of Yimeng black lambs, while 15 d after weaning can accelerate the generation of short-chain fatty acids in the cecal contents of lambs, promote the colonization of beneficial bacteria such as Bacillus and Ruminococcus after 35 d of weaning, and improve the metabolism of nutrients of lambs.