从巨噬细胞极化角度分析不同油脂饮食对小鼠腹股沟皮下脂肪组织沉积的影响

为分析两种脂肪供能条件下3种不同油脂饮食对小鼠腹股沟皮下脂肪沉积及其巨噬细胞分型的影响，90只C57BL/6J小鼠被随机分为6组，分别饲喂低脂猪油（Lar-10%）、低脂菜籽油（Rap-10%）、低脂橄榄油（Oli-10%）、高脂猪油（Lar-30%）、高脂菜籽油（Rap-30%）和高脂橄榄油（Oli-30%）,16周后解剖取腹股沟皮下脂肪组织，并对其进行苏木精-伊红染色，活性氧（Reactive oxygen species,ROS）含量检测，M1、M2型巨噬细胞标记物（CD11c和CD206）荧光双染，并通过ELISA检测其含量。结果表明：1）在低脂饮食条件下，三种油脂对小鼠腹股沟皮下脂肪组织重量、脂肪细胞横截面积、腹股沟皮下脂肪ROS含量均无显著影响（P>0.05）;2）随着脂肪供能水平的提高，小鼠腹股沟皮下脂肪沉积显著增加（P<0.05），在高脂饮食条件下，Lar-30%组的小鼠腹股沟皮下脂肪组织重量显著高于Rap-30%和Oli-30%组（P<0.05），并且相对于其他两组，Oli-30%组小鼠腹股沟皮下脂肪组织中ROS含量极显著降低（P<0.01）;...

Effects of different fat diets on inguinal subcutaneous adipose tissue deposition in mice from the perspective of macrophage polarization

The study explored the effects of three different oil and fat diets on inguinal subcutaneous fat deposition and macrophage typing in mice under two kinds of fat energy supply conditions. A total of 90 C57BL/6 J male mice were randomly divided into 6 groups, and fed with low-fat lard(Lar-10%), low-fat rapeseed oil(Rap-10%), low-fat olive oil(Oli-10%), high-fat lard(Lar-30%), high-fat rapeseed oil(Rap-30%), and high-fat olive oil(Oli-30%). After 16 weeks, the inguinal subcutaneous adipose tissues were dissected and stained by hematoxylin-eosin staining, reactive oxygen species(ROS)content was detected. M1and M2 macrophage markers(CD11c, CD206)were double stained with fluorescence, and their contents were detected by ELISA. The results showed that: 1)Under the condition of low-fat diet, the three kinds of oil/fat diets had no significant differences on inguinal subcutaneous adipose tissues weight, its cross-sectional area, and ROS content in subcutaneous adipose tissues(P>0. 05); 2)With the increase of fat energy supply, inguinal subcutaneous fat deposition increased significantly(P<0. 05). Under the condition of high-fat diet, the weight of inguinal subcutaneous adipose tissue in Lar-30% group was significantly higher than that in Rap-30% and Oli-30% groups(P<0. 05), and the content of ROS in inguinal subcutaneous adipose tissue in Oli-30% group was significantly lower than that in the other two groups(P<0. 01); 3)High-fat diet increased content of CD11c and CD206, macrophage markers, in adipose tissue, but the ratio of CD206/CD11c in mice fed olive oil was significantly higher than that in mice fed lard and rapeseed oil(P<0. 05). In brief, reducing the level of fat energy can effectively reduce fat deposition. Compared with lard and rapeseed oil, olive oil diet may reduce fat accumulation by increasing the ratio of CD206/CD11c, inhibiting the transformation of macrophages to M1 type, reducing the production of ROS in adipose tissue to reduce inflammation.