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Glycoproteomic profiling of serum peptides in canine lymphoma and transitional cell carcinoma |
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| Authors: | C R Wilson F E Regnier D W Knapp R E Raskin D A Andrews S B Hooser |
| Institution: | Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN, USA; Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN, USA; Department of Chemistry, Purdue University, West Lafayette, IN, USA; Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN, USA |
| Abstract: | Differential expression of fucosylated glycoproteins has been correlated with malignancy and metastatic potential in various types of neoplasia. Utilizing glycoproteomics techniques, changes in fucosylated serum peptides associated with naturally occurring canine lymphoma and transitional cell carcinoma (TCC) have been evaluated. In both types of neoplasia, the majority of the fucosylated peptides that changed increased with the cancer. In one lymphoma case that was examined over the course of the disease, the same fucosylated peptides that increased during pre-chemotherapy decreased during post-chemotherapy, and then subsequently increased upon recurrence of the lymphoma. When comparing all the fucosylated peptides that increased in both types of cancer, there were only two peptides in common allowing discrimination between lymphoma and TCC based on their peptide profiles. These results emphasize the prospect of glycopeptide profiling in proteomics for use in discovering a panel of non-invasive, diagnostic or prognostic biomarkers of cancer. |
| Keywords: | bladder cancer cancer glycosylation lectin lymphoma proteomics |