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| 番茄花药离体培养中低温预处理对小孢子发育的影响 | |
| 引用本文： | 周广栋,王秀峰,谢 冰,张德安.番茄花药离体培养中低温预处理对小孢子发育的影响[J].中国农学通报,2005,21(2):192-192. |
| 作者姓名： | 周广栋 王秀峰 谢 冰 张德安 |
| 作者单位： | 1. 山东农业大学园艺科学与工程学院,泰安,271018 2. 山东农业大学科技学院,泰安,271018 3. 临沂市林业科学研究所,8242021 |
| 基金项目： | 山东省农业良种产业化开发项目(鲁科农字[2001]500号). |
| 摘 要： | 为明确低温预处理对番茄花药离体培养过程中小孢子发育的影响，进行了番茄花药离体培养的低温预处理试验，试验结果表明：离体培养条件下，雄核发育存在三条途径，即B途径、A-V途径和A-G途径，以B途径为主；随着培养时间的增加，小孢子逐渐退化；对番茄花药离体培养进行低温预处理，可在一定程度上延缓番茄小孢子退化，提早雄核发育，并增加参与雄核发育小孢子的比率，但参与B-途径的小孢子比参与A-途径的增加得多。 |
| 关 键 词： | 番茄 花药 离体培养 低温预处理 小孢子 |
The effect of cold pretreatment on microspores development during in vitro culture of tomato anther |
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| Zhou Guangdong,Wang Xiufeng,Xie Bing,Zhang Dean.The effect of cold pretreatment on microspores development during in vitro culture of tomato anther[J].Chinese Agricultural Science Bulletin,2005,21(2):192-192. | |
| Authors: | Zhou Guangdong Wang Xiufeng Xie Bing Zhang Dean |
| Institution: | Zhou Guangdong1,Wang Xiufeng1,Xie Bing,Zhang De'an2 |
| Abstract: | we carried on cold pretreatment experiment during tomato anther culture to study the effect of cold pretreatment on microspores development during tomato anther culture in vitro. The results indicated that: Pathway B、A-V，and A-G, existed in androgenesis of tomato of which pathway B was predominent. Degeneration happened with some pollen; Cold pretreatment could delay degeneration of microspores to a certain extent and advance androgenesis; Cold pretreatment could also enhance the ratio of microspores participated in androgenesis, however pathway B increased greater than pathway A; In the early stage of culturing, cold pretreatment could increase the ratio of uninucleate microspores, which gave more chance for microspores to participate in androgenesis. |
| Keywords: | Tomato(Lcopersion esculentum Mill ) Anther In vitro culture Cold pretreatment Microspores |
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