水稻种子贮藏蛋白总含量分布和多态性分析

用考马斯亮蓝G 250染色法测定了来自世界各地的110个水稻品种或品系种子贮藏蛋白的含量，发现不同水稻品种或品系种子贮藏蛋白含量存在一定的多样性。110个水稻材料贮藏蛋白含量分布在34.29 ~95.08 mg/g，含量最高的为来自国际水稻研究所的品系C105A51；大部分水稻材料的种子贮藏蛋白含量在40 ~60 mg/g，有72个，占所测水稻材料总数的655%；总蛋白质含量超过80 mg/g的水稻材料很少，只有3个，占2.7 %。SDS PAGE蛋白电泳图谱表明水稻贮藏蛋白种类和含量存在较高的多态性。

Total Content and Polymorphism of Seed Storage Proteins in Rice

Rice seed storage protein contents in 110 rice varieties or lines were determined by using the staining method with Comassie brilliant blue G-250.There were obvious diversities of seed storage protein contents among the varieties or lines tested.The contents of rice seed storage proteins ranged from 34.29 to 95.08 mg/g,but 66.3% varieties or lines were in the range of 40 to 60 mg/g.The rice varieties or lines with more than 80 mg/g of rice seed storage protein contents were rare,only accounting for 2.7% of the total tested materials.The result of SDS-PAGE for seed storage proteins also displayed obvious polymorphisms among the rice varieties or lines.It could provide useful information for seed storage protein analysis and for rice breeding.