对节白蜡树叶乙酸乙酯提取物的GC-MS分析及其结构初探

为了研究对节白蜡的抗病原因,以探寻新的生物农药,在前期有关对节白蜡树叶提取物杀菌活性研究的基础上,对其乙酸乙酯提取物及柱层析分离组分进行了GC-MS分析,初步确认了各组分所含化合物的可能结构。结果表明:乙酸乙酯提取物中面积归一含量超过1%的化合物组分有18个,其中3号化合物的面积归一含量为17.13%,其结构为4-羟苯基乙醇;经过柱层析分离后各组分所含化合物要相对集中和更加纯化些,其中组分A所含物质主要有两个,化合物4-羟苯基乙醇的含量达到了81.3%,这是相对比较纯的物质;作者据其已有的相关研究结果(组分A整体表现出的杀菌活性要优于其他组分)分析认为,4-羟苯基乙醇作为对节白蜡树叶中所含的重要化合物,在对节白蜡的抗病方面起到了重要作用。

GC-MS analysis and preliminary study on structures of extracts from Fraxinus hupehensis leaves

In order to study the anti-disease cause of Fraxinus hupehensis in search of a new bio-pesticide,on the basis of the early bactericidal activity researches on extract from F.hupehensis leaves,ethyl acetate extracts and its column chromatography isolation ingredients were analyzed by GC-MS,and the possible structures of compounds contained in each ingredient were preliminarily identified.The results showed that,in ethyl acetate extracts,area normalization contents of 18 compound ingredients were over 1％,among which the No.3 compound ingredient had area normalization contents of 17.13％ and had the structure of 4-hydroxyl phenyl ethanol.Through column chromatography isolation,compounds contained in each ingredient were more concentrated and purer.Ingredient A contained two substances,and its 4-hydroxyl phenyl ethanol content reached 81.3％,which was relatively pure.According to available relevant research results,the bactericidal activity shown by Ingredient A was better than that of the others.So 4-hydroxyl phenyl ethanol,as a important compound in F.hupehensis leaves,played an important role in the anti-disease ability ofF.hupehensis.