猪SOCS-2基因cDNA全长序列的克隆与组织表达

根据Genbank已登录的细胞因子信号转导抑制因子(SOCS-2)基因序列同源区设计1对引物,从8月龄健康雄性八眉猪肾脏组织中提取总RNA,利用RT-PCR技术扩增猪SOCS-2基因;用CLUSTAL W软件进行同源性分析,利用半定量(Semi-quantitative,SQ)RT-PCR技术检测八眉猪各组织中SOCS-2 mRNA的表达量。结果表明,猪SOCS-2基因cDNA全长克隆成功(Genbank登录号为EF121242);家猪SOCS-2基因与澳洲野猪、人、小鼠、大鼠SOCS-2核苷酸同源性分别为99%,94%,91%,89%,SOCS-2蛋白氨基酸序列同源性分别达到100%,95%,94%,93%;SOCS-2基因在心脏、肺、皮下脂肪组织、腹部脂肪组织、肝脏、脾、肾脏和肌肉组织中广泛表达,其中肾脏组织中最高,心脏次之,肌肉组织中表达量最低。表明猪SOCS-2序列保守性较高,在体内可能具有多种功能。

Full cDNA cloning and tissue distribution of SOCS-2 gene in pig

SOCS-2 was very important to growth and development of organism and growth hormone signaling.A pair of primers were designed according to the homology region of SOCS-2 among other species.Total RNA was isolated from kidney of Bamei pig,a local strain of Chinese pig,and the complete cDNA sequence of SOCS-2 gene was cloned by RT-PCR technology firstly(Genbank accepted number is EF121242).Thereafter,the distribution of SOCS-2 mRNA in Bamei pig was analyzed by SQ-RT PCR.Compared with others,the homology of SOCS-2 to Sus scrofa,human,mouse and rat was 99%,94%,91% and 89%,respectively,but the homology of protein reached 100%,95%,94% and 93%,respectively.And the data showed that the SOCS-2 could be detected in many tissues extensively,such as heart,lung,subcutaneous adipose,abdominal adipose,liver,spleen,kidney and muscle.Its expression was higher in kidney and heart but lower in muscle tissue.