首页 | 本学科首页   官方微博 | 高级检索  
     

番茄斑萎病毒属病毒S RNA序列比对
引用本文:徐小刚,刘雅婷. 番茄斑萎病毒属病毒S RNA序列比对[J]. 湖南农业大学学报(自然科学版), 2010, 36(2): 142-146. DOI: 10.3724/SP.J.1238.2010.00142
作者姓名:徐小刚  刘雅婷
作者单位:云南农业大学,农学与生物技术学院,云南,昆明,650201;云南农业大学,烟草学院,云南,昆明,650201
基金项目:国家973计划项目,云南省自然科学基金项目,云南省教育厅项目 
摘    要:从生物信息数据库NCBI中搜索并下载最新有关番茄萎斑病毒属Tospovirus各种间S RNA片段全长序列以及该片段上NSs、N的核酸和蛋白质序列着手,运用DNAstar和DNAMAN以及NPSA等生物信息软件对其进行比对分析,结果发现,以NSs蛋白序列建立的系统进化树显示CCSV和TZSV之间的同源性为85.8%,IYSV和TYRV之间的同源性达90%,MYSV和PSMV间的同源性为97.5%;通过N蛋白序列分析显示,Tospovirus属中有7组同源性较高的种;在Tospovirus属病毒中非极性氨基酸含量最高,极性酸性氨基酸最少,比较TSWV中抗性破坏RB株系与普通株系在NSs、N核酸、蛋白质序列、氨基酸含量和蛋白质二级结构之间的差异,发现差异不显著.

关 键 词:番茄斑萎病毒属  核壳体序列  SRNA上的非结构蛋白序列  抗性破坏
收稿时间:2009-09-02
修稿时间:2010-01-06

The alignment of S RNA sequences in Tospovirus
xu xiao gang and liu ya ting. The alignment of S RNA sequences in Tospovirus[J]. Journal of Hunan Agricultural University, 2010, 36(2): 142-146. DOI: 10.3724/SP.J.1238.2010.00142
Authors:xu xiao gang and liu ya ting
Affiliation:Yunnan Agricultural University,College of Agricultural and Biotechnology ,Yunnan Agricultural University, Kunming
Abstract:The nucleic acid and protein sequences on NSs, N and S RNA full-length sequences of Tospovirus genus were downloaded from NCBI database, and they were aligned by bio-informatics software DNAstar, DNAMAN and NPSA. It was demonstrated that homology between CCSV and TZSV was 85.8% on homologous phylogenetic tree on NSs protein sequences, homology between IYSV and TYRV reached 90%, homology between MYSV and PSMV was up to 97.5%. There are 7 groups of high homology with analysing the N protein sequences. Tospovirus is rich in non-polar amino acids but polar-acid amino acid is the lowest. At the same time, by alignment TSWV strains between resistance-breaking strain and wild strains on NSs, N nucleic acid and protein sequences, amino acids and the protein secondary structure with no significant differences found.
Keywords:Tospovirus   nucleocapsid protein sequences   non-structural protein in the S RNA full-length sequence   resistance-breaking
本文献已被 万方数据 等数据库收录!
点击此处可从《湖南农业大学学报(自然科学版)》浏览原始摘要信息
点击此处可从《湖南农业大学学报(自然科学版)》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号