| 板栗雄花序石蜡切片改进方法的研究 |
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| 引用本文： | 韩宝,冯永庆,秦岭. 板栗雄花序石蜡切片改进方法的研究[J]. 安徽农学通报, 2008, 14(3): 29-30 |
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| 作者姓名： | 韩宝 冯永庆 秦岭 |
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| 作者单位： | 北京农学院植物科学技术系,北京,102206;北京农学院植物科学技术系,北京,102206;北京农学院植物科学技术系,北京,102206 |
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| 基金项目： | 北京市自然科学基金，北京农业职业学院校科研和教改项目 |
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| 摘 要： | 本文以板栗雄花序为实验材料进行石蜡制片,通过改良透明、包埋试剂、染色等制片环节,建立了一种快速石蜡制片方法.该法简化了实验步骤、将制片周期缩短为原来的1/3,其制得的切片质量同常规方法无区别,并减少了实验过程中有毒试剂的使用量,整体上提高了制片效率.
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| 关 键 词： | 板栗 雄花序 石蜡制片法 |
| 文章编号： | 1007-7731（2008）03-29-02 |
| 收稿时间： | 2008-01-03 |
| 修稿时间： | 2008-01-03 |
Improvement of Paraffin Method in Chestnut Catkins |
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| Han Bao,Feng Yongqing,Qin Ling. Improvement of Paraffin Method in Chestnut Catkins[J]. Auhui Agricultural Science Bulletin, 2008, 14(3): 29-30 |
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| Authors: | Han Bao Feng Yongqing Qin Ling |
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| Abstract: | In the paper,the chestnut catkins were used in paraffin slice.The links of clearing and imbedding agent were optimized;a rapid paraffin method was established.The process was simplified and the whole experimental time had been cut two-third.The quality of slices used the rapid paraffin method was no differentiation into common method.Simultaneously it reduced the quantity of harmful chemical agent,and improved the efficiency and quality of making slices. |
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| Keywords: | Chestnut Catkins Paraffin method |
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