鸡精原干细胞分离培养初步研究 |
| |
引用本文: | 采克俊,刘莉,张易祥,于立颖,丁海雷,丁志丽,谢小丹.鸡精原干细胞分离培养初步研究[J].安徽农业科学,2008,36(34). |
| |
作者姓名: | 采克俊 刘莉 张易祥 于立颖 丁海雷 丁志丽 谢小丹 |
| |
作者单位: | 1. 湖州师范学院生命科学学院,浙江湖州,313000 2. 扬州大学动物科学与技术学院,江苏扬州,225009 |
| |
基金项目: | 国家自然科学基金,浙江省科技攻关重点项目,湖州市自然科学基金 |
| |
摘 要: | 目的]建立禽类精原干细胞(SSCs)体外培养系统。方法]以25日龄广西土鸡为试材,通过睾丸石蜡切片和HE染色,了解SSCs发育情况,用两步酶消化法分离获得生精上皮细胞,比较不同温度、接种密度对鸡SSCs体外培养的影响,并对SSCs的集落进行染色鉴定。结果]结果显表明,25日龄时SSCs已经形成,但还未分化。37℃适宜作为鸡SSCs的培养温度,5×105个/ml适宜作为鸡SSCs原代混合培养的接种密度。经碱性磷酸酶和糖原染色鉴定,体外培养的鸡SSCs仍保持未分化的特性。结论]该研究为禽类SSCs体外培养的基础资料提供参考。
|
关 键 词: | 鸡 精原干细胞 分离 培养 鉴定 |
Preliminary Study on the Isolation and in vitro Culture of Chicken Spermatogonial Stem Cells |
| |
Abstract: | Objective] The aim of study was to found in vitro culture system of SSCs for birds kind.Method] 25-day-old male chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion.The spermatogonial stem cells(SSCs) development stage was evaluated by histological examination after hematoxylin-eosin staining.The effects of different culture temperatures and concentration on primary culture of chicken SSCs were evaluated,and the SSCs colonies were characterized by staining.Result]The results showed that by 25-days,SSCs,as the most advanced germ cells,were present,but undifferentiated.The temperatures at 37 ℃ and seeding at a concentration of 5×105 cells/ml were suitable for chicken SSCs culture.During in vitro culture,the colony-forming SSCs were stained positively for alkaline phosphatase and periodic acid schiff solution,demonstrating that these cells still remained undifferentiated in this culture system.Conclusion]This study will provide the reference for basic data of in vitro culture of bird kind SSCs. |
| |
Keywords: | Chicken Spermatogonial stem cells Isolation Culture in vitro Characterization |
本文献已被 维普 万方数据 等数据库收录! |
|