Influence of storage on soil microbial biomass estimated by three biochemical procedures |
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Authors: | D.J. Ross K.R. Tate Annette Cairns Karen F. Meyrick |
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Affiliation: | Soil Bureau, Department of Scientific and Industrial Research, Lower Hutt, New Zealand |
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Abstract: | The effects of 28 and 56 days' storage at 25°, 4° and ?20°C on the microbial biomass content of four soils from tussock grasslands were studied by three biochemical procedures. Two of the procedures involved measurement of CO2 and mineral-N (Min-N) production by chloroform-fumigated and unfumigated soil, and consequent estimation of biomass C and Min-N flush respectively. In the third, adenosine 5'-triphosphate (ATP) content was determined.Patterns of CO2 production were often influenced by storage treatment. The use of fixed incubation periods for estimating the CO2 flush of fumigated soil and the steady rate of CO2 production by unfumigated soil did, however, give biomass C estimates that were generally similar to those calculated from individually determined incubation periods for each treatment and soil.Biomass C values could change significantly at all storage temperatures, but generally least at ?20°C. Storage at ?20°C was also the most suitable for retaining ATP contents, whereas 4°C was best for values of Min-N flush. Values of Min-N flush after storage of soil at ?20°C decreased significantly in two of the soils but increased in another. No storage temperature was thus satisfactory for all three indices of microbial biomass. Generally, however, 4°C was adequate for short periods, and 25°C the least suitable. |
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