首页 | 本学科首页   官方微博 | 高级检索  
     


Extraction,purification and properties of soil hydrolases
Authors:L. Batistic  J.M. Sarkar  J. Mayaudon
Affiliation:Laboratoire d''Ecologie Microbienne, Faculté des Sciences Agronomiques Université de Louvain, Place Croix du Sud, 2, 1348-Louvain-la-Neuve, Belgium
Abstract:Invertase, cellulase, phosphatases, protease and β-glucosidase were extracted from permanent pasture soil with 0.2 M phosphate buffer (pH 8) in the presence of 0.2 M EDTA. This extract was further treated with ammonium and salmine sulphates. Attempts were made to fractionate these enzyme activities by gel and anion-exchange chromatography. Specific activities were estimated in all fractions and some characteristics of the purified enzymes (optimum pH, temperature and substrate concentration, and Km and Vmax) were investigated. The results indicated that extracted enzyme activities occurred partly in soil as a carbohydrate-enzyme complex and partly as a humo-carbohydrate complex.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号