| 柞蚕幼虫与蛹期DNA提取分析 |
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| 引用本文： | 张洋,万军,郝大东,靳向东,薛强,朱兴友. 柞蚕幼虫与蛹期DNA提取分析[J]. 北方蚕业, 2015, 0(1): 1-5. DOI: 10.3969/j.issn.1673-9922.2015.01.001 |
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| 作者姓名： | 张洋 万军 郝大东 靳向东 薛强 朱兴友 |
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| 作者单位： | 吉林省蚕业科学研究院,吉林吉林,132112 |
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| 基金项目： | 现代农业产业技术体系建设专项 |
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| 摘 要： | 为探讨柞蚕DNA的最佳提取方法,以蚕体不同发育时期的组织材料为研究对象,提取DNA后进行比较分析。结果发现,利用五龄蚕足部血液的冻存材料为样品,加入4倍提取液,抽提的DNA质量较好,且样品无需液氮研磨,减少了消化时间,提高了工作效率,节约了成本。
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| 关 键 词： | 柞蚕 基因组DNA 提取 |
Different Periods of Tissue DNA Extraction and Analysis of Antheraea pernyi |
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| ZHANG Yang,WAN Jun,HAO Dadong,JIN Xiangdong,XUE Qiang,ZHU Xingyou. Different Periods of Tissue DNA Extraction and Analysis of Antheraea pernyi[J]. North Sericulture, 2015, 0(1): 1-5. DOI: 10.3969/j.issn.1673-9922.2015.01.001 |
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| Authors: | ZHANG Yang WAN Jun HAO Dadong JIN Xiangdong XUE Qiang ZHU Xingyou |
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| Affiliation: | ZHANG Yang;WAN Jun;HAO Dadong;JIN Xiangdong;XUE Qiang;ZHU Xingyou;Jilin Provincial Sericultural Research Institute; |
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| Abstract: | In order to get the best method for extraction of Tussah DNA ,this research took Antheraea pernyi's tissue mate‐rial from different periods to extract DNA and do comparative analysis .It is found that adding 4 times of extraction to cryopreserved material of foot blood from 5th instar silkworms can produce the best DNA .Without liquid nitrogen grind‐ing ,it reduces the digestion time ,improves work efficiency ,and saves cost as well . |
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| Keywords: | Antheraea pernyi Genomic DNA extraction |
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