雷公藤叶片基因组DNA不同提取方法比较 |
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引用本文: | 邢建宏,刘希华,庞铄权,梁一池. 雷公藤叶片基因组DNA不同提取方法比较[J]. 安徽农业科学, 2010, 38(11): 5553-5555 |
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作者姓名: | 邢建宏 刘希华 庞铄权 梁一池 |
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作者单位: | 三明学院化学与生物工程系,福建三明,365004;福建中医学院,福建福州,350108 |
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基金项目: | 福建省教育厅A类科技项目 |
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摘 要: | [目的]比较雷公藤叶片中基因组DNA的提取方法。[方法]采用常规CTAB法、改良CTAB法、常规SDS法、高盐低pH值法和一步法分别提取雷公藤叶片基因组DNA,利用琼脂糖凝胶电泳、紫外分光光度法测定样品OD260与OD280的比值以及RAPD扩增结果,判断所得DNA样品的纯度,并根据OD260值计算不同提取方法的DNA得率。[结果]改良CTAB法提取效果最佳,所提DNA的平均得率为219.3μg/g,OD260/OD280比值在1.869~1.903,用于RAPD-PCR均有较好的扩增结果。[结论]改良CTAB法是雷公藤叶片基因组DNA提取的最佳方法。
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关 键 词: | 雷公藤 DNA提取 不同提取方法 |
Comparasion on Several Extraction Methods of Genomic DNA from Tripterygium wilfordii Hook.f.Leaves |
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Affiliation: | XING Jian-hong et al (Department of Chemical and Biological Engineering of San Ming University,Sanming,Fujian 365000) |
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Abstract: | [Objective] Comparison of the extraction methods of genomic DNA from the leaves of Tripterygium wilfordii Hook.f..[Method] With leaves of Tripterygium wilfordii Hook.f.as tested material,the genomic DNA was extracted using five different methods:CTAB method,modified CTAB method,SDS method,high salt and low pH method and ROSE method.The purity of the DNA was examined by agarose gel electrophoresis,OD260/OD280 and RAPD amplification,and the yield of the DNA was estimated by OD260.[Result] Modified CTAB method was the optimum one for DNA extraction.The ratio of OD260 and OD280 of the genomic DNA extracted by modified CTAB method was 1.869-1.903,and showed good performance in RAPD analysis.[Conclusion]The modified CTAB method is an optimum method for the extraction of genomic DNA from Tripterygium wilfordii Hook.f.. |
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Keywords: | Tripterygium wilfordii Hook.f.. DNA extraction Different extraction methods |
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