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利用分子标记改良水稻白叶枯病抗性的研究
引用本文:倪大虎,李莉,吴家道,向太和,汪秀峰,张毅,杨剑波,许传万,贾士荣,张世平. 利用分子标记改良水稻白叶枯病抗性的研究[J]. 安徽农业科学, 2001, 29(6): 697-698,700
作者姓名:倪大虎  李莉  吴家道  向太和  汪秀峰  张毅  杨剑波  许传万  贾士荣  张世平
作者单位:1. 安徽省农业科学院水稻研究所,
2. 中国农业科学院生物技术研究所
3. International,Laboratory,for,tropiced,Agricultural,Biotechnology
基金项目:国家转基因植物研究和产业化专项基金;2JY-B-02-01;
摘    要:利用分子标记技术对转抗白叶枯病基因 (Xa2 1)的明恢 63和 80 4B后代进行跟踪筛选 ,得到了纯合稳定株系。同时对转基因恢复系明恢 63进行配组试验 ,对转基因 80 4B纯合稳定系进行不育系的转育 ,并对其配组和转育后代进行PCR分析和人工接菌 ,结果表明Xa2 1基因能稳定遗传表达 ,并出现了一些既抗白叶枯病又有增产潜力的组合

关 键 词:分子标记辅助选择  Xa21基因  抗白叶枯病水稻

Study on the Improvement of Rice with the Resistance to Bacterial Bl ight through the Method of Molecular Marker Assisted Selection
Li Dahu et al. Study on the Improvement of Rice with the Resistance to Bacterial Bl ight through the Method of Molecular Marker Assisted Selection[J]. Journal of Anhui Agricultural Sciences, 2001, 29(6): 697-698,700
Authors:Li Dahu et al
Abstract:The stable transgenic rice-Minhui 63 and 80-4B with Xa21 gene that had the resistance to rice bacterial blight was obtained with the method of marker assisted selection after gene transformation. The combining test of the transgenic restorer Minhui 63 and sterile line transformation of transgenic 80-4B had been done. Xa21 gene was confirmed in F1 of combinations and sterile line through the PCR assay and artificial inocubation. The resistant character of Xa21 gene can be stably passed to the offspring and some combinations with both strong resistance and high yield potential were selected.
Keywords:Molecular marker assisted selection   Xa21 gene   Rice with the resistance to bacterial blight
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