人工栽培铁皮石斛与其相似种的SRAP分子标记分析 |
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引用本文: | 李杰,王再花.人工栽培铁皮石斛与其相似种的SRAP分子标记分析[J].中国农学通报,2016,32(25):79-83. |
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作者姓名: | 李杰 王再花 |
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作者单位: | 广东省农业科学院环境园艺研究所,广东省农业科学院环境园艺研究所 |
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基金项目: | 广东省农业科学院院长基金项目(201417);“十二五”农村领域国家科技计划课题“热带兰种业关键技术研究”(2012BAD01B0702);广东省科技计划项目“观赏玫瑰石斛的开发研究”。 |
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摘 要: | 利用SRAP分子标记对搜集的35份资源(24份人工栽培铁皮石斛、9份石斛属植物、石斛伪品石豆兰和石仙桃各1份)的遗传多样性及亲缘关系进行分析。从30对SRAP引物中筛选出10对多态性引物,共获得90条多态性条带,多态性比率98.9%。SRAP标记检测结果显示,来源不同的人工栽培铁皮石斛的遗传多样性非常丰富。UPGMA聚类分析可将上述样品分为8类,其中24份铁皮石斛资源聚为一簇,而与其他石斛和石斛伪品明显分开,1份未知石斛经验证为铁皮石斛和滇桂石斛的杂交种。
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关 键 词: | 苏禽绿壳蛋鸡 苏禽绿壳蛋鸡 开产性状 全期产蛋性状 体尺性状 典型相关 |
收稿时间: | 2016/1/18 0:00:00 |
修稿时间: | 8/3/2016 12:00:00 AM |
SRAP Analysis of Cultivated Dendrobium officinale and Similar Species |
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Abstract: | The genetic diversity and relationship of 35 germplasm resources (24 cultivated Dendrobium officinale, 9 Dendrobium species and 2 adulterants) were analyzed by SRAP markers. Ten SRAP primers were screened for the SRAP-PCR reaction, and 91 polymorphic bands were detected, and the polymorphic percentage was 98.9%. The SRAP results indicated the genetic diversity of cultivated D. officinale from different areas was rich. UPGMA analysis showed the 35 germplasm resources divided into 8 groups, 24 cultivated D. officinale were in one group, and separated from other Dendrobium species and adulterants. It also showed that unknown hybrid was a cross between D. officinale and D. guangxiense. |
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Keywords: | Dendrobium officinale Similar species SRAP |
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