| EMS诱变小麦愈伤组织选择抗旱突变体的研究 |
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| 作者姓名： | 王 瑾 刘桂茹 杨学举 |
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| 作者单位： | 河北农业大学农学院，河北农业大学农学院，河北农业大学生命科学学院 河北保定 071001，河北保定 071001，河北保定 071001 |
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| 基金项目： | 国家自然科学基金资助项目(30271075)
河北省自然科学基金资助项目(303184) |
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| 摘 要： | 以抗旱性差的小麦品种温麦6号和周麦17的花药愈伤组织和幼胚愈伤组织为材料,进行 EMS诱变处理,结果表明:EMS溶液处理花药愈伤组织相对分化率近50％的浓度和时间组合为 0．20％ (2-6h);处理幼胚愈伤组织相对分化率近50％的浓度和时间组合为(0．20％-0．40％) (2-4h)。 将EMS诱变处理后分化的224株再生植株接种到PEG浓度为80g/L的生根培养基中,进行抗旱筛 选,共得到13株抗旱植株,平均变异率为5．8％。花药愈伤组织和幼胚愈伤组织EMS溶液诱变处理的 适宜浓度和时间组合为0．20％ 4h。
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| 关 键 词： | 小麦 愈伤组织 EMS诱变 PEG胁迫 抗旱突变体 |
Callus Mutation with EMS and Drought-resistant Mutants Selection for Wheat |
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| Authors: | Wang Jin Liu Guiru Yang Xueju |
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| Institution: | 1 College of Agronomy, Agricultural University of Hebei, Baoding Hebei 071001; 2College of Life Science, Agricultural University of Hebei, Baoding, Hebei 07100 |
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| Abstract: | Anther callus and immature embryos callus from drought- sensitive wheat (Wen6 and Zhou17) were investigated using EMS as mutagens. About 50% relative differentiation rate dose of the mutagens were used as the selective indexes of optimal mutagen concentrations and treatment time. The combination of treatments were as follows: Anther callus treatment with EMS solution 0.20% (2h-4h); Immature embryos callus treatment with EMS solution (0.20%-0.40%) (2-4h). The 224 differentiation plants were planted in the rooting medium added 80g/L PEG, which were differentiated from EMS mutate materials, and 13 drought resistance plants were got. EMS mutation ratio was 5.8%. The best combination of treatment were callus treatment with EMS solution 0.20% 4h. |
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| Keywords: | Wheat Callus EMS mutation PEG water-stress Drought resistance mutant |
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