| 利用基因工程创造植物雄性不育的策略 |
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| 作者姓名： | 杨泽良 党选民 曹振木 胡开林 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所 海南省儋州571737
(杨泽良，党选民，曹振木)，华南农业大学园艺学院 广东省广州510642(胡开林) |
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| 基金项目： | 国家自然科学基金资助项目(30370978)，国家科技基础条件平台项目(2004KD30420)资助。 |
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| 摘 要： | 利用基因工程创造植物雄性不育系是近年来发展的新技术。根据花粉败育的原理,利用花药细胞绒毡层、绒毡层线粒体、胼胝质壁、细胞减数分裂过程等花粉形成过程中的不同环节,采取外源细胞毒素基因、分子伴侣、反义RNA技术、提早降解胼胝质、扰乱线粒体与细胞核之间的信息交流和细菌影响花粉小孢子发育等策略,使花粉不能正常形成,从而产生不育花粉,达到产生雄性不育的目的。并提出了基因工程创造植物雄性不育系可能存在的问题及其应用前景。
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| 关 键 词： | 基因工程 雄性不育 杂种优势 |
The Application of Gene Engineering on Creating Male Sterility in Plants |
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| Authors: | Yang Zeliang Dang XuanMin Cao Zhenmu Hu Kailin |
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| Institution: | 1.Tropical Crops Genetic Resource Institute, Chinese Academy of Tropical Agricultural Science, Zhanzhou Hainan, 571737; 2. Horticultural College, South China Agricultural University, Guanzhong Guangdong, 510642 |
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| Abstract: | Genetic engineering is a new way of creating male sterility in plants. Based on principle of pollen abortion, utilizing tapetum of anther cell, mitochondria of tapetum, callose well, process of cell meiosis during formation process of pollen, we adopt genetic transformation to create plant male sterility, such as, exogenous cytotoxin gene, chaperones, anti-sense RNA, degradation of callose ahead of time, disturbing information intercommunion between mitochondria and nucleolus and affected development of pollen sporule by bacteria. The paper also brought forward some potential disadvantages and application prospect on application of gene engineering on creating male sterility in plants. |
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| Keywords: | Gene engineering Male sterility Heterosis utilization |
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