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IGFBP3基因多态性与鲁西牛和晋南牛部分屠宰性状的相关性
作者姓名:党瑞华  魏伍川  陈 宏  蓝贤勇  胡沈荣  苏利红
作者单位:西北农林科技大学动物科技学院,湛江师范学院生物系,西北农林科技大学动物科技学院,西北农林科技大学动物科技学院,西北农林科技大学动物科技学院,西北农林科技大学动物科技学院 陕西省农业分子生物学重点实验室,杨凌712100,广东湛江524048,陕西省农业分子生物学重点实验室,杨凌712100徐州师范大学生命科学学院生物技术研究所,徐州221116,陕西省农业分子生物学重点实验室,杨凌712100,陕西省农业分子生物学重点实验室,杨凌712100,陕西省农业分子生物学重点实验室,杨凌712100
基金项目:国家863计划(2002AA242011,2003AA243051)资助.
摘    要:利用PCR-RFLP技术对24头鲁西牛和19头晋南牛的IGFBP3基因多态性及与屠宰性状的关系进行了研究。结果表明,鲁西牛和晋南牛IGFBP3基因存在多态性,但只有AA、AB2种基因型,基因型频率分别为0.875/0.125和0.895/0.105,等位基因A/B的频率分别为0.938/0.062和0.948/0.052。对IGFBP3基因座基因型与19个屠宰性状进行了分析,在鲁西牛群体中,除胴体深、日增重、净肉率、屠宰率4个指标外,其它指标AB型均好于AA型,但差异不显著;在晋南牛群体中,除屠宰率、骨重、膘度、胴体胸深、后腿围、后腿宽、后腿长、腰部肉厚这8个指标外,其它指标AA型均好于AB型,差异也不显著。

关 键 词:鲁西牛  晋南牛  IGFBP3基因  PCR-RFLP  屠宰性状

Polymorphisms of Insulin-like Growth Factor Binding Protein 3 Gene and Its Associations with Several Carcass Traits in Luxi and Jinnan Cattle
Authors:Dang Ruihu  Wei Wuchuan  Chen Hong  Lan Xianyong  Hu Shenrong  Su Lihong
Institution:(1College of Animal Science and Technology, Northwest Sci- Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling 712100; 2Biology college, Zhanjiang Normal University, Zhanjiang, Guangdong, 524048;
Abstract:PCR-RFLP technique was used to analyze the relationship between the polymorphisms of IGFBP3 gene and beef performance in 24 Luxi cattles and 19 Jinnan cattles. The results showed that there were polymorphisms in this locus, but only AA and AB genotypes. The genotype frequencies were 0.875/0.125 and 0.895/0.105 respectively. A and B gene frequencies were 0.938/ 0.062 and 0.948/0.052 respectively. Correlations between IGFBP3 genotypes and 19 beef performances were analyzed, and other indexes of individuals with genotypes AB were higher than those of AA except carcass depth, daily gain, net meat percentage, dressing percentage in Luxi cattle, which had no significant difference; While other indexes of individuals with genotypes AA were higher than those of AB except dressing percentage, bone weight, backfat thickness, carcass chest depth, ham girth, ham width, ham length, loin meat thickness in Jinnan cattle, which were insignificant.
Keywords:Luxi cattle  Jinnan cattle  IGFBP3 gene  PCR-RFLP  Beef performance
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