| 不同培养基和激素浓度处理对草地
早熟禾愈伤组织诱导的影响 |
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| 作者姓名： | 段碧华 韩宝平 高遐虹 尹伟伦 |
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| 作者单位： | 北京农学院植物科技系 102206
(段碧华，韩宝平，高遐虹)，北京林业大学生物学院 100083(尹伟伦) |
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| 基金项目： | 北京市科技新星项目资助(H020821300120). |
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| 摘 要： | 以3种不同的草地早熟禾种子为外植体,研究了不同培养基、不同浓度的2,4-D、BA及水解酪蛋白对愈伤组织诱导的影响。试验表明:(1)MN6培育基有利于愈伤组织的诱导;(2)种胚愈伤组织的诱导和继代宜采用MN6 2,4-D3mg/L BA0.2mg/L的培养基;(3)水解酪蛋白对草地早熟禾种子愈伤组织诱导的影响不大;(4)3种草地早熟禾种子中,新哥莱德(Nuglade)出愈率较高。
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| 关 键 词： | 培养基 激素浓度 草地早熟禾 愈伤组织 |
Effect of Different Culture Medium and Hormone Concentration on Callus Induction of Poa Pratensis L |
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| Authors: | Duan Bihu Han Baoping Gao Xiahong Yin Weilun |
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| Institution: | (1Department of Plant Science in Beijing Agricultural College, 102206; 2College of Biology Science in Beijing Forestry University, 100083) |
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| Abstract: | Study was carried out on effect of different concentration acetic acid(2,4-D),6-benzylamino purine(6-BA)and casein hydroly-sate(Hy)under different culture medium on callus induction of three varieties of Poa Pratensis L.with its seeds as explants.The result showed that (1)MN6 culture medium could improve callus induction rate;(2)culture medium of callus induction and regeneration of embryo was MN6 2,4-D 3mg/L BA0.2mg/L;(3)effect of different concentration casein hydroly-sate(Hy) was weak on callus induction;(4) callus induction rate of Nuglade was the highest in three varieties of Poa Pratensis L. |
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| Keywords: | Poa pransis L Callus induction rate Hormone concentration Culture medium |
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