干旱条件下玉米耐旱相关性状的QTL一致性图谱构建

 发掘玉米耐旱基因及其连锁分子标记是构建耐旱分子育种技术的重要基础。通过生物信息学手段整理玉米基因组数据库中已有耐旱相关性状的QTL信息,借助高密度玉米分子标记连锁图谱IBM和临近分子标记,建立耐旱相关性状的QTL一致性图谱,进而发掘通用QTL。研究显示,在干旱条件下于10个定位群体中共发现与9个玉米农艺及生理性状有关的181个QTL,建立了全长3582.1cM的耐旱相关性状的QTL一致性图谱,发掘出15个"通用耐旱QTL"及其连锁标记。研究结果为确定玉米耐旱基因位点和建立分子标记辅助育种技术创造了条件。

Consensus Map of the QTL Relevant to Drought Tolerance of Maize Under Drought Conditions

Discovery of drought tolerant genes and their closely linked markers could provide useful information for setting molecular breeding strategy. In this paper, QTL relevant to drought tolerance in maize genomic database were explored, and the consensus map was constructed with the aid of high-density linkage map IBM and the adjacent markers to each QTL. Consequently, a total of 181 QTL involving in the expression of 9 morhpho-physiological traits in 10 mapping populations of maize under drought conditions were screened out. The length of the consensus map constructed was 3582.1cM. Furthermore, 15 "universal drought tolerance QTL" and their linked markers were identified. The results will facilitate to determine the genetic loci and molecular marker-assisted selection strategy for drought tolerance in maize.