粗皮桉的组织培养与植株再生研究

目的]研究不同植物生长调节剂组合对粗皮桉茎段愈伤组织诱导及再生的影响,为建立粗皮桉再生体系和粗皮桉商品化育苗提供参考.方法]以粗皮桉茎段为外殖体,通过对不同植物生长调节剂组合的优化,建立粗皮桉再生体系.结果]在MS培养基上添加2.0 mg/L PBU和0.05 mg/L IAA,21d后,外植体愈伤组织诱导率达98.4％;将愈伤组织接种在添加0.8 mg/L 6-BA和0.1 mg/L NAA的1/2MS培养基上,诱芽率高达86.0％;用1/2MS培养基添加0.8 mg/LPBU与0.1 mg/L NAA诱导粗皮桉不定芽增殖,用1/2MS培养基附加0.5 mg/L IBA和0.5 mg/L NAA诱导生根,炼苗15d后,组培苗移栽到黄心土与腐殖质土质量比为1∶1的基质中,成活率高达95.0％.结论]2.0 mg/L PBU和0.05 mg/L IAA是诱导粗皮桉愈伤组织的最适植物生长调节剂组合,添加0.8 mg/L 6-BA和0.1 mg/L NAA的1/2MS培养基诱芽效果最好,添加0.1 mg/L NAA 、0.8 mg/L PBU组合时,芽增殖、生长情况较好,添加0.5 mg/L NAA 、0.5 mg/L IBA组合时生根效果最佳.

Tissue culture and regeneration of Eucalyptus pellita

Objective]The effects of different growth regulator combinations on callus induction and regeneration of Eucalyptus pellita were studied to provide references for establishing Eucalyptus pellita regeneration system and cultivating commercial seedlings. Method ]Using Eucalyptus pellita stems as explants, the regeneration system was established by comparing the variety of combinations of different plant growth regulators. Result]MS medium supplemented with the syn- thetic growth regulator 2 mg/L PBU and 0.05 mg/L IAA. After cultivation for 21 d, 98.4% of explants formed callus. Callus were transferred to MS medium containing 0.8 mg/L 6-BA and 0.1 mg/L-NAA to induce bud formation, and 86.0% of cal- lus exhibited adventitious bud formation. Proliferation of adventitious buds was then stimulated on I/2MS supplemented with 0.8 mg/L PBU and 0.1 mg/L NAA for 20 d. For rooting, the elongated shoots were cultivated on root induction medi- um containing 0.5 mg/L IBA and 0.5 mg/L NAA. After seedling stress for 15 days, the survival rate reached 95.0% with a mixture of red-heart soil and humus soil （1：1 by volume） as a substrate for transplanting. Conclusion]The plantlet regen- eration system of Eucalyptus pellita was successfully established by comparing the variety of combinations of different plant growth regulators. The optimum combinations of plant growth regulators for inducing the callus of Eucalyptus pellita were 2 mg/L PBU and 0.05 mg/L IAA. The optimum combinations of plant growth regulators for inducing the bud differentiation of Eucalyptus pellita were 0.8 mg/L 6-BA and 0.1 mg/L NAA. The optimum combinations of plant growth regulators for in- ducing buds proliferation of Eucalyptus pellita were 0.10 mg/L NAA and 0.8 mg/L PBU. The optimum combinations of plant growth regulators for inducing rooting were 0.5 mg/L NAA and 0.5 mg/L IBA.