库页红景天多糖的抗氧化作用评价

【目的】探讨库页红景天多糖的体外抗氧化作用，为红景天多糖的合理开发利用提供科学依据。【方法】采用黄嘌呤—黄嘌呤氧化酶—鲁米诺发光体系、抗坏血酸-CuSO4-酵母-H2O2系统及荧光法分别测定库页红景天多糖对超氧阴离子（O2）、羟自由基（·OH）、NO的清除作用，同时采用Fe2＋诱发脂蛋白过不饱和脂肪酸（PUFA）过氧化体系评价库页红景天多糖的抗脂质过氧化作用。【结果】库页红景天多糖的总糖含量为42.49%，糖醛酸含量为38.28%。库页红景天多糖能有效清除O2、·OH、NO及抑制脂质过氧化，抑制率分别达到90.50%、87.72%、70.25%和85.05%，其对应的IC50分别为3.30 μg/mL、0.90 mg/mL、20.00 μg/mL和0.90 mg/mL。【结论】库页红景天多糖是一种良好的天然抗氧化剂，可应用于食品、医疗和保健等方面。

Antioxidation of polysaccharide from Rhodiola sachalinensis

【Objective】This research aimed to investigate antioxidation in vitro of Rhodiola sachalinensis polysaccharide （RSP）, in order to provide scientific references for its reasonable utilization. 【Method】Xanthine-xanthine oxidase-luminol glow system, ascorbic acid-CuSO4-yeast-H2O2 system and fluorescence method were employed to determine the scavenging action of RSP on superoxide anion, ·OH and NO radical. The Fe2＋-induced lipoprotein unsaturated fatty acid peroxidation system were used to evaluate anti-lipid peroxidation of RSP. 【Result】The total and uronic acid content of RSP was 42.49% and 38.28%, respectively. RSP could effectively scavenge superoxide anion, hydroxyl and nitric oxide radical, and inhibit lipid peroxidation with the inhibition rates of 90.50%, 87.72%, 70.25% and 85.05%, respectively, and the IC50 values were 3.30, 0.90, 20.00 and 0.90 mg/mL, respectively. 【Conclusion】RSP, an excellent natural antioxidant, could be used for food, medical treatment and healthcare.