广西优良珍贵树种灰木莲的组织培养

【目的】探索灰木莲组培快繁体系,为灰木莲的进一步开发和利用提供参考依据。【方法】以灰木莲盆栽苗的带芽茎段为外植体,以MS为基本培养基,通过添加不同种类、不同浓度植物生长调节剂,研究不同培养基对腋芽萌发、芽增殖及生根的影响。【结果】1～5号培养基均可以诱导灰木莲腋芽萌发,其中3号培养基MS＋6-BA0.5mg/L＋NAA0.1mg/L腋芽萌发速度最快,芽的诱导率89.3%;添加KT（Kinetin,激动素）的10～13号培养基增殖系数为2.2～2.7,没有添加KT的6～9号培养基增殖系数为1.8～2.2,11号培养基MS＋6-BA0.3mg/L＋NAA0.05mg/L＋KT1.0mg/L芽的增殖系数可达2.7,为最适宜的增殖培养基;添加生长调节剂浓度在0.5mg/L以上的17～21号培养基能诱导灰木莲组培苗生根,其中NAA浓度为0.5、1.5、2.0mg/L生根率分别为33.3%、45.4%、58.3%,21号培养基1/2MS＋NAA2.0mg/L上生根率最高。【结论】初步建立灰木莲组织培养体系,组培苗的芽诱导率和增殖率较高,苗木长势良好。

Tissue culture of rare species Manglietia glauca in Guangxi

Objective Rapid regeneration system for Manglietia g1auca was explored to provide references for its further development and utilization, seedling replacement and tissue culture of other magnoliaceae plants. Method The tissue culture of Manglietia g1auca was studied using stems with buds as explants. The explants were cultivated in minimal medium with different types and concentrations of plant growth regulators. ResultThe axillary bud of Manglietia g1auca explants could be induced in 1-5 medium. The best shoot induction medium was 3 （MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L） with the highest induction rate of 89.3%. The multiplication coefficient of plantlets in 10-13 medium added with kinetin （KT） were 2.2-2.7, while the coefficients in 6-9 medium without KT were 1.8-2.2. With the highest multiplication coefficient of 2.7, the optimum medium for multiplication was 11 （MS＋6-BA 0.3 mg/L＋NAA 0.05 mg/L＋KT 1.0 mg/L）. The roots of plantlets could be induced on medium 17-21 with the concentration of NAA higher than 0.5 mg/L. The rooting rate were 33.3%, 45.4% and 58.3%, while NAA in the medium were 0.5, 1.5 and 2.0 mg/L, within which the best rooting medium was 21 （1/2MS＋NAA 2.0 mg/L）. Conclusion The regeneration system of Manglietia g1auca was basically established. The plantlets grew well because of high induction rate and high propagation coefficient. The rooting induction should be further studied.