抗真菌转基因水稻种植对土壤有机氮组分的影响

【目的】探讨转基因水稻对土壤有机氮组分的影响，为评价转基因作物对土壤供氮能力的影响提供依据。【方法】采用田间小区试验，按抗真菌转基因水稻品种设转品1、转品8两个处理及其亲本非转基因水稻七丝软粘作对照处理，于水稻播种前及收获后采集土样测定分析土壤有机氮组分及真菌数目变化情况。【结果】转基因水稻转品1、转品8和亲本非转基因水稻七丝软粘的土壤有机氮组分在种植前和收获后均无明显差异，外源抗真菌基因的引入对水稻田土壤有机氮各组分含量及其所占全氮比例无明显的影响，土壤有机氮中酸解氮的各组分含量顺序为：氨基酸态氮〉酸解未知氮〉氨态氮〉氨基糖态氮。【结论】外源抗真菌基因的引入对土壤有机氮组分的影响差异不显著。

Effects of antifungal transgenic rice on organic nitrogen composition in soil

Objective]Effects of transgenic rice on soil organic nitrogen composition were studied to provide refer- ences for evaluating the role of transgenic crops in nitrogen supplying capability of soil. Method ] Field plot experiment was carried out, two antifungal transgenic rice varieties viz., Zhuanpin 1 and Zhuanpin 8 were tested by using non-GMO par- ents Qisiruanzhan as control （CK）, and organic nitrogen composition and fugal number variation of soil samples were deter- mined before sowing and after harvesting. Result ]The results showed that there was no remarkable difference in organic nitrogen compositions among the three treatments before planting or after harvest. Organic nitrogen compositions and pro- portion of total nitrogen were not significantly influenced by exogenous antifungal genes. The contents of soil acid hy- drolyzed N components in descending order was as follows： amino acid N〉hydrolyzed unidentified N〉ammonium N〉amino sugar N. The contents of amino acid N and amino sugar N were highly aflhcted by the number of soil fungi which directly impacted the degradation ability of soil organic matter. Conclusion]Exogenous antifungal genes had little influence on or- ganic nitrogen fractions.