马槟榔组培快繁技术研究

目的]探讨不同灭菌剂对外植体的灭菌效果及不同激素组合对马槟榔芽诱导和增殖的影响,建立马槟榔的组培快繁体系.方法]用马槟榔一年生的幼嫩茎段及幼苗作为外植体,以MS为基本培养基,附加不同的激素组合对外植体进行芽诱导和增殖培养.结果]相对于10％次氯酸钠,0.1％升汞对马槟榔外植体灭菌效果更理想,灭菌率达到90％,且外植体的生长状况良好.在芽诱导培养中,随着6-BA的增加,马槟榔芽的诱导数呈先增后减的变化趋势;当6-BA为1.0 mg/L时,诱导率最高;当NAA为0.5 mg/L时,诱导率相对较高.在增殖培养中,当6-BA用量大于NAA时,增殖系数较低;当6-BA用量小于NAA时,增殖系数较高,最高可达3.8,且丛芽长势好,芽苗粗壮,叶片颜色正常.结论]升汞对马槟榔外植体的灭菌效果优于次氯酸钠;芽的最佳诱导培养基为MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L,芽增殖最佳培养基为MS＋6-BA 0.5 mg/L＋NAA 1.0 mg/L.

Tissue culture and rapid propagation technique of Capparis masaikai

Objective]The rapid propagation and culture technique system of Cappads masaikai was established by studying the effects of different disinfectants on explants and different concentrations and combinations of hormone on in- duction and multiplication of buds. Method ]Adventitious buds and multiplication were induced using the one-year-old tender shoot, segments and plant as explants in basic MS medium containing different hormone combinations. Result] Mercuric-chloride showed better effects than sodium hypochlorite on the disinfection of explants. The sterilization rate was 90%, and the explants grew well. The induction number of adventitious buds increased first but fell later with the increase of 6-BA concentration in induction medium of bud. The germination rate was the highest when 6-BA concentration was 1.0 mg/L, the induction rate was higher when NAA was 0.5 mg/L. When 6-BA concentration was greater than NAA concen- tration, multiplication coefficient was lower, and when 6-BA concentration was less than NAA concentration, multiplica- tion coefficient was higher with the maximum of 3.8. The cluster buds grew better. The plantlets were thick, and the leaf color was normal. Conclusion]The sterilization effect of mercuric chloride was better than that of sodium hypoehlorite. The optimal medium recipe for inducing buds and multiple shoots of Cappads masaikai was MS＋I.0 mg/L 6-BA＋0.5 mg/L NAA and MS＋0.5 mg/L 6-BA＋I.0 mg/L NAA, respectively.