激素对花生根愈伤组织诱导的影响

【目的】通过试验优化激素配比，获得花生根愈伤组织诱导率高的激素组合。【方法】以花生根组织为原料，采用三元二次通用旋转方法设计试验，将花生根组织接种在含不同配比的激素培养基中进行诱导，并统计其诱导率。【结果】三元二次通用旋转方法分析结果表明，回归模型R^2为0．9521，所获回归模型可较好拟合愈伤组织诱导率与各参试因子之间的关系，并达到极显著水平，二次回归数学模型为：y=83．399-1．335x1-2．156x2＋5．469x3-1．844x1x2-1．259x1^2-1．940x2^2-3．557x3^2。单因子分析结果表明，诱导率与2，4-D、KT、6-BA之间均呈抛物线关系，随着激素浓度水平的增加，诱导率表现为先增加后下降的趋势；2，4-D、KT、6-BA的最佳诱导浓度分别为2．04、1．67、3．05mg／L。2，4-D、KT和6-BA的交互作用明显，当2，4-D、KT和6-BA3种激素组合浓度分别为2．50、1．80、3．00mg／L时，花生愈伤组织诱导率达最高值，为87．55％。【结论】不同激素种类对花生根愈伤组织诱导率的影响程度为6-BA〉KT〉2，4-D。以MS为基本培养基，附加30g／L蔗糖、9g／L琼脂及不同浓度配比的2．50mg／L2，4-D、1．80mg／LKT、3．00mg/L6-BA，对花生根愈伤组织诱导具有明显促进效果。

Effects of hormone on callus induction of peanut root

Objective]Hormone proportion was optimized to get the best combination for the callus induction of peanut root. Method]Using quadratic rotation combinatorial design, peanut root tissue was inoculated to medium under different hormone combinations and its corresponding callus induction rate was statistically analyzed. Result ] The analysis showed that the regression model with R2=0.9521 could best fit the relations in callus induction rate and its effective factors and could reach the extremely significant level, and the quadratic regression model was as follows：y=83.399-1.335x1- 2.156x2＋5.469x3-1.844x1x2-1.259x12-1.940xz2-3.557x32. n single factor analysis, callus induction rate was parabolically re- lated with 2,4-D, KT and 6-BA. Along with the increase of hormone concentration, callus induction rate was increased first and then decreased; the best concentrations for 2,4-D,KT and 6-BA were 2.04,1.67 and 3.05 mg/L,respectively. 2,4-D, KT and 6-BA actively interacted with each other. When the concentration of 2,4-D, KT and 6-BA was 2.5,1.8 and 3.0 mg/L, the callus induction rate of peanut root was the highest （87.55%）. Conclusion ]The order of the effect of three hormones on callus induction rate of peanut root was 6-BA〉KT〉2,4-D. Callus induction of peanut root could be greatly promoted cultured in MS medium added with 30 g/L sucrose, 9 g/L agar, 2.5 mg/L 2,4-D, 1.8 mg/L KT, and 3.0 mg/L 6-BA.