花烛愈伤组织不同继代培养的再分化差异

 对花烛( Anthurium andraeanum) ‘Jolanba’无菌苗叶片诱导产生的愈伤组织进行3 种不同的培养处理, 观察其再分化表现, 并探讨了3 种继代培养愈伤组织的生理生化特点。结果表明, 适当降低基本培养基浓度、减少继代次数和延长继代周期培养的愈伤组织分化的芽较多、较壮; 再生芽的分化需要较高水平的可溶性蛋白质, 其生长需要较高水平的可溶性糖; POD 和SOD 活性与分化的芽数呈正相关; GA_{1 + 3}/ABA、ZR_s/ ABA 的高比例与再生芽的高度呈显著正相关; IAA/ ABA 及IAA 的高水平与花烛离体根的发生呈正相关。重新诱导培养和延长愈伤组织继代周期以提高再生芽生长势的主要生理生化基础之一是维持较高的可溶性糖水平和有利的激素平衡。

Relative Physiological and Biochemical Features of Redifferentiation Difference in Three Types of Calli Subculture in Anthurium andraeanum

Calli of Anthurium andraeanum‘Jolanba’derived from leaves of in vitro plantlets were cultured with three different treatments to observe their redifferentiation behaviors , and their physiological and biochemical features are discussed. The results went as followed. Decrease of base medium concentration , reduction of sub-culture frequency , or prolongation of sub-culture period favored differentiation and growth of regenerated plantlets.High content of soluble protein favored shoot differentiation , and high content of soluble sugar enhanced shoot growth. There was obvious positive correlation between activities of POD and SOD and number of differentiated shoots. So was between GA_{1 + 3}/ ABA , as well as ZR_s/ ABA , and shoot height . The IAA/ ABA ratio or IAA was also related to rhizogenesis. One of the physiological and biochemical causes for the enhancement growth vigor of aseptic plantlet by renewing in vitro culture system and prolonging culture period was maintaining high content of endogenous soluble sugar and favorable balance of endogenous phytohormes.