| 两种桃芽总RNA提取方法的比较研究 |
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| 引用本文： | 王宇,李玲,王慧,陈修德,高东升. 两种桃芽总RNA提取方法的比较研究[J]. 山东农业大学学报(自然科学版), 2011, 42(3): 388-391 |
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| 作者姓名： | 王宇 李玲 王慧 陈修德 高东升 |
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| 作者单位： | 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018 |
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| 摘 要： | 以‘曙光油桃’芽为试材,采用改良CTAB法、Trizol法进行总RNA提取,并通过凝胶电泳、核酸蛋白分析仪和Q-PCR检测所提取总RNA样品的质量。结果表明:改良CTAB法提取的RNA,经Q-PCR后扩增桃芽HK基因,可以得到与目的片段大小一致的条带,条带清晰,能满足基因表达分析等后续试验的要求;而Trizol法所提取的RNA,条带较弱,经Q-PCR后扩增桃树芽内HK基因,得不到与目的片段大小一致的条带,难以达到进一步试验的要求。
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| 关 键 词： | 曙光油桃 芽 总RNA 提取 |
COMPARISON STUDY ON TWO MODIFIED EXTRACTING METHODS FOR PEACH BUDS TOTAL RNA |
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| WANG Yu,LI Ling,WANG Hui,CHEN Xiu-de,GAO Dong-sheng. COMPARISON STUDY ON TWO MODIFIED EXTRACTING METHODS FOR PEACH BUDS TOTAL RNA[J]. Journal of Shandong Agricultural University, 2011, 42(3): 388-391 |
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| Authors: | WANG Yu LI Ling WANG Hui CHEN Xiu-de GAO Dong-sheng |
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| Affiliation: | WANG Yu,LI Ling,WANG Hui,CHEN Xiu-de,GAO Dong-sheng*(State Key Laboratory for Crop Biology/College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an 271018,China) |
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| Abstract: | The bud of peach(Prunus persica var.nectariana cv.Shuguang) was used as material for extracting total RNA with Modified CTAB method and Trizol extraction kit.The quality of the total RNA was tested by gel electrophoresis,nuclear acid/protein analyzer and Q-PCR.The results showed that the RNA got from CTAB method had a better quality with a distinct bands,and using this RNA to amplify HK gene in peach bud by Q-PCR can obtain the target fragments,and could satisfy the requirement of molecular biology trial.Ho... |
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| Keywords: | Peach bud total RNA extraction |
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