5年生种植人参对成年小鼠免疫器官、细胞吞噬功能及自然杀伤细胞的影响

目的探讨不同工艺制备的人参食用对成年小鼠免疫器官、细胞吞噬功能及自然杀伤细胞的影响。方法正常成年ICR小鼠随机分为10组,即:对照组,5年生种植人参原粉小、中、大剂量(0.25、0.5、1.0g生药/kg)组,5年生种植人参水提物小、中、大剂量(0.25、0.5、1.0g生药/kg)组,5年生种植人参醇提物小、中、大剂量(0.25、0.5、1.0g生药/kg)组,每组20只,连续灌胃给药1个月后,每组分别处死10只小鼠、2各月后每组再处死10只小鼠。观察各组小鼠胸腺指数和脾脏指数、巨噬细胞吞噬功能、自然杀伤(NK)细胞的杀伤率。结果水提取物、乙醇提取物大剂量显著增加正常成年小鼠的胸腺指数和脾脏指数;水提取物、乙醇提取物大、中剂量显示出提高巨噬细胞对鸡红细胞(CRBC)的吞噬率和吞噬指数及小鼠自然杀伤细胞对L929细胞的杀伤率的趋势。结论人参对于免疫器官重量及指数、腹腔巨噬细胞吞噬率及吞噬指数、自然杀伤细胞率有一定的提高作用。

The Effect of 5-year-old planting ginseng on immune function in adult mice

Objective To observe the effect of 5-year-old planting ginseng on immune function in mice. Methods Normal adult ICR mice were randomly divided into 10 groups： control group, the low, medium, high dose groups of original powder of S-year-old planting ginseng （0.25, 0.5 and 1.0g crude drug/kg）; the low, medium, high-dose groups of water extract from S-year-old planting ginseng （0.25, 0.5 and 1.Og crude drug/kg）; the low, medium, high-dose group of ethanol extract from 5-year-old planting ginseng （0.25, 0.5 and 1.0 g crude drug/kg）, ten mice for each group. Immune function was observed after the mice were administered orally one consecutive month, two consecutive months. Results Compared with the control group, the high dose or medium dose group of water or ethanol extract from ginseng is tendency in improving the percentage of Proliferation of spleen cells and serum IgG. The production of IL-2 and INF-yof supernatant of spleen cell culture were significantly higher in high dose group of water or ethanol extract from ginseng （P〈0.05）. Conclusion water or ethanol extract from ginseng showed the Enhance activity for body immune fuction.