| 两系杂交稻亲本SSR指纹图谱的建立及其在种子纯度鉴定中的应用 |
| |
| 引用本文： | 李进波,方宣钧,杨国才,费震江,戚华雄. 两系杂交稻亲本SSR指纹图谱的建立及其在种子纯度鉴定中的应用[J]. 杂交水稻, 2005, 20(2): 50-53 |
| |
| 作者姓名： | 李进波 方宣钧 杨国才 费震江 戚华雄 |
| |
| 作者单位： | 1. 湖北省农业科学院,杂交水稻工程技术研究中心,湖北,武汉,430064
2. 海南省热带农业资源开发利用研究所,海南,三亚,572025 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 利用筛选到的20对SSR引物建立了两系杂交稻32个亲本(24个光温敏不育系和8个恢复系)的DNA指纹图谱,针对所涉及的9个杂交稻组合,获得能在父、母本间表现出多态性的特异SSR标记48个.以杂交稻组合两优932为例,在实验室用一个特异SSR标记(RM302)对200粒种子样本进行了纯度鉴定,结果鉴定纯度为90.50%,与田间种植鉴定结果90.60%(海南鉴定)和91.57%(武汉鉴定)基本一致,表明SSR标记技术适用于构建DNA指纹图谱和种子纯度鉴定.
|
| 关 键 词： | 两系杂交水稻 SSR(微卫星标记) 指纹图谱 种子纯度鉴定 |
| 文章编号： | 1005-3956(2005)02-0050-04 |
| 修稿时间： | 2004-06-07 |
SSR Fingerprinting the Parents of Two-line Hybrid Rice and Its Application in Purity Identification of Hybrid Seeds |
| |
| LI Jin-bo,FANG Xuan-jun,YANG Guo-Cai,FEI Zhen-jiang,QI Hua-Xiong. SSR Fingerprinting the Parents of Two-line Hybrid Rice and Its Application in Purity Identification of Hybrid Seeds[J]. Hybrid Rice, 2005, 20(2): 50-53 |
| |
| Authors: | LI Jin-bo FANG Xuan-jun YANG Guo-Cai FEI Zhen-jiang QI Hua-Xiong |
| |
| Abstract: | In this research, DNA fingerprints of 24 male sterile lines and 8 restorer lines of two-line hybrid rice were constructed by using 20 SSR (Simple sequence repeats) primers. The 20 SSR primers could amplify 70 polymorphic bands totally with a mean of 3.5 bands per primer. 48 specific SSR markers for identifying the seed purity of 9 two-line hybrid rice combinations were acquired.The 200 single seeds of Liangyou 932 were tested by a specific SSR marker (RM302) in laboratory. The seed purity was 90.50%, which was approximate to the field purity (90.60% in Hainan and 91.57% in Wuhan, respectively). The results of this research suggested that SSR technique could be used in DNA fingerprinting construction and seed purity identification of two-line hybrid rice. |
| |
| Keywords: | two-line hybrid rice SSR (Simple sequence repeats) fingerprint purity identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
