薏苡不同种质的基因组原位杂交分析

为了研究薏苡属不同种质之间的关系,采用基因组原位杂交(GISH)技术,以四倍体栽培薏苡(2n=20,AABB)基因组总DNA作为探针,分别对广西的栽培薏苡、野生薏苡和水生薏苡体细胞中期染色体进行基因组原位杂交。杂交结果显示,栽培薏苡和野生薏苡染色体都被强烈而密集的杂交信号所标记,说明野生薏苡与栽培薏苡在基因组染色体水平上的同源程度很高,保守重复序列占很大比重;水生薏苡的基因组中有20条染色体的DNA成分与栽培薏苡的基因组DNA高度同源,推断供试的水生薏苡种属于广西六倍体水生薏苡居群。

Analysis of different Jobstears Coix species with genomic in situ hybridization

Genomic in situ hybridization(GISH)method was used to study the relationship of different Jobstears Coix species,and the cell metaphase chromosomes of Coix Lacryma-jobi L,Coix Agrestis Lour and Coix Aquatica Roxb were hybridized with labelled probes genome from Coix Lacryma-jobi L.(AABB genomes).The GISH results indicated that the chromosomes of Coix Lacryma-jobi L and Coix Agrestis Lour was labelled with strong and intensive hybridization signals,which showed that the homology between Coix Lacryma-jobi L and Coix Agrestis Lour genomes was very close at genomic chromosomes level,and the conservative repeated sequences accounted for a large proportion.Moreover,the DNA components of 20 chromosomes from Coix Aquatica Roxb genome was highly homologic with the genome DNA of Coix Lacryma-jobi L.It is concluded that testing Coix aquatica Roxb belongs to hexaploid Coix aquatica Roxb population in Guangxi.