| 双梯度超薄胶PAGE分离DNA及其银染与回收 |
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| 引用本文： | 朱新产,王宝维,张涌.双梯度超薄胶PAGE分离DNA及其银染与回收[J].西北农业学报,2002,11(1):20-23. |
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| 作者姓名： | 朱新产 王宝维 张涌 |
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| 作者单位： | 1. 莱阳农学院动科系分子生物学研究室,山东莱阳,265200
2. 西北农林科技大学,陕西杨凌,712100 |
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| 基金项目： | 山东省三O工程(9969);莱阳农学院基金(2003)资助项目. |
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| 摘 要： | 双浓度梯度超薄胶PAGE分离DDRT－PCR扩增产物表明，在30cm长的凝胶板上能清晰分辨长度仅差1bp的DNA，清楚地显示100多条分离谱带，并可直接从银染PAGE胶中回收DNA谱带。简便节省，是基因表达分析的有效方法。
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| 关 键 词： | 双梯度超薄胶PAGE DNA 分离 银染 回收 DDRT－PCR |
| 文章编号： | 1004-1389(2002)01-0020-04 |
| 收稿时间： | 2001/12/11 0:00:00 |
| 修稿时间： | 2001年12月11 |
DNA Fragments Separating by Double-gradient Super-thin PAGE and Recovering from Silver-stained Gels |
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| ZHU Xin-chan,WANG Bao-wei and ZHANG Yong.DNA Fragments Separating by Double-gradient Super-thin PAGE and Recovering from Silver-stained Gels[J].Acta Agriculturae Boreali-occidentalis Sinica,2002,11(1):20-23. |
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| Authors: | ZHU Xin-chan WANG Bao-wei and ZHANG Yong |
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| Institution: | Lab of Mol Biology, Dept of Veterinary Science LaiYang Agricultural College, LaiYang ShanDong 265200, China;Lab of Mol Biology, Dept of Veterinary Science LaiYang Agricultural College, LaiYang ShanDong 265200, China;Lab of Mol Biology, Northwestern Agricultural University, Yangling Shaanxi 712100, China |
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| Abstract: | DDRT-PCR amplification products were separated in double-gradient super-thin PAGE.The results showed that single base-pair (bp) difference between two DNAs was clearly detected in 30 cm long gels, and over 100 bands were com pletely separated by the double-gradient PAGE.On the other hand the objective bands could be directly recovered from silver-stained PAGE gels.The method is simply, economical, and makes it possible availably to analyes gene xpression. |
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| Keywords: | DDRT-PCR |
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