| 四倍体青贮玉米染色体加倍及检测技术研究 |
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| 引用本文： | 齐欣,姜敏,马骏,刘欣芳,弓雪,李明,王延波. 四倍体青贮玉米染色体加倍及检测技术研究[J]. 玉米科学, 2016, 24(1): 28-32 |
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| 作者姓名： | 齐欣 姜敏 马骏 刘欣芳 弓雪 李明 王延波 |
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| 作者单位： | 辽宁省农业科学院玉米研究所, 沈阳110161,辽宁省农业科学院玉米研究所, 沈阳110161,辽宁省农业科学院玉米研究所, 沈阳110161,辽宁省农业科学院玉米研究所, 沈阳110161,辽宁省农业科学院玉米研究所, 沈阳110161,辽宁省农业科学院玉米研究所, 沈阳110161,辽宁省农业科学院玉米研究所, 沈阳110161 |
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| 基金项目： | 辽宁省外专局项目(四倍体青贮玉米研发)、辽宁省科技厅项目(2014201003,201501112)、国家转基因重大专项(2014ZX08003-004,2014ZX08003-003)、国家自然科学基金项目(31401392) |
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| 摘 要： | 以辽单527、辽单青贮178、辽原一号3个玉米品种为试验材料,通过6个浓度的秋水仙素溶液处理,进行染色体加倍,采用流式细胞仪检测加倍效果。结果表明,当秋水仙素浓度达0.1%时,3个品种加倍率均达到最高值,辽单527的加倍效果最好,最高可达36.3%。利用流式细胞仪能够快速准确检测染色体倍性,OTTO法制备悬浮液的检测效果较好。
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| 关 键 词： | 玉米 秋水仙素 染色体检测 |
| 收稿时间： | 2015-03-13 |
Research on Doubling Chromosome and Ploidy Identification of Silage Maize |
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| QI Xin,JIANG Min,MA Jun,LIU Xin-fang,GONG Xue,LI Ming and WANG Yan-bo. Research on Doubling Chromosome and Ploidy Identification of Silage Maize[J]. Journal of Maize Sciences, 2016, 24(1): 28-32 |
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| Authors: | QI Xin JIANG Min MA Jun LIU Xin-fang GONG Xue LI Ming WANG Yan-bo |
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| Affiliation: | Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China,Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China,Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China,Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China,Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China,Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China and Maize Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China |
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| Abstract: | In order to detect the double effect by flow cytometry(FCM), there varieties of silage maize including Liaodan527, Liaodan178 and Liaoyuan No.1 were chosen as the materials, and 6 concentrations of colchicine were used for chromosome doubling. The results showed that, the doubling rate were all reached the highest level when the concentration of colchicine was 0.1%, and that of Liaodan527 was the best, which was 36.3%; FCM could correctly detect the chromosome multiple and OTTO method had better effect. |
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| Keywords: | Maize Colchicine Chromosome detection |
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