| 黄瓜棒孢叶斑病病原菌RFP标记转化株的构建 |
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| 引用本文： | 谢学文,张自心,武军,石延霞,柴阿丽,李宝聚.黄瓜棒孢叶斑病病原菌RFP标记转化株的构建[J].中国蔬菜,2018,1(3):45-49. |
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| 作者姓名： | 谢学文 张自心 武军 石延霞 柴阿丽 李宝聚 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京100081 |
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| 基金项目： | 国家自然科学基金项目（31401888），国家大宗蔬菜产业技术体系项目（CARS-25） |
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| 摘 要： | 由多主棒孢(Corynespora cassiicola)侵染引起的黄瓜棒孢叶斑病已成为我国黄瓜生产上的重要新流行病害,目前尚缺乏抗性品种,病原菌侵染机制及与寄主的互作关系尚不清楚。为了开展多主棒孢的病原学研究,本试验采用农杆菌基因介导技术(ATMT),获得了红色荧光蛋白(RFP)标记的多主棒孢转化株,转化株在PDA培养基转接6次后仍能在菌丝和分生孢子上发出强烈的红色荧光,生物学测定和致病性测定结果表明该转化株与野生型菌株无显著差异。
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| 关 键 词： | 黄瓜棒孢叶斑病 多主棒孢 农杆菌介导法 RFP |
Construction of RFP Transformation as a Marker in Corynespora cassiicola from Cucumber |
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| XIE Xue-wen,ZHANG Zi-xin,WU Jun,SHI Yan-xia,CHAI A-li,LI Bao-ju.Construction of RFP Transformation as a Marker in Corynespora cassiicola from Cucumber[J].China Vegetables,2018,1(3):45-49. |
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| Authors: | XIE Xue-wen ZHANG Zi-xin WU Jun SHI Yan-xia CHAI A-li LI Bao-ju |
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| Institution: | Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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| Abstract: | Cucumber Corynespora leaf spot caused by Corynespora cassiicola has become an important new epidemic disease in cucumber production in China．At present，there is no resistant varieties and the infection mechanism of C. cassiicola and its interaction with host are still unknown．In order to investigate the pathogen of C. cassiicola，the RFP-labeled transformant was generated with Agrobacterium tumefaciens-mediated transformation（ATMT）．The transformant was able to emit strong red fluorescence on mycelia and conidia during 6 passages of transformants on PDA．The biological determination and pathogenicity test demonstrated that there was no significant differences between the wild strain and the transformant． |
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| Keywords: | Cucumber Corynespora leaf spot Corynespora cassiicola Agrobacterium mediated RFP |
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