| 蒙古马高负荷运动训练前后MSTN基因、CKM基因表达量分析 |
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| 引用本文： | 康德措,其日格日,赵启南,赵一萍,芒来. 蒙古马高负荷运动训练前后MSTN基因、CKM基因表达量分析[J]. 中国畜牧杂志, 2018, 0(1) |
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| 作者姓名： | 康德措 其日格日 赵启南 赵一萍 芒来 |
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| 作者单位： | 内蒙古农业大学动物科学学院内蒙古自治区蒙古马遗传资源保护及马产业工程实验室; |
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| 摘 要： | 为探讨蒙古马高负荷调教训练前后MSTN、CKM基因的表达情况,实验首先采用SYBR GreenⅠ荧光定量RT-PCR方法对4个常用内参基因(TTN、18s r RNA、GAPDH、β-actin)在臀中肌内的稳定性进行评估;确定内参基因后,对MSTN、CKM基因转录水平进行测定。结果表明:在蒙古马臀中肌中稳定度依次为GAPDH(1.087)β-actin(1.211)TTN(1.285)18s r RNA(1.460),选取稳定度最高的GAPDH基因作为内参基因;高负荷运动训练后MSTN基因m RNA表达量较训练前上调,而CKM基因则下调。本研究为蒙古马运动性能相关基因的研究奠定了基础。
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| 关 键 词： | 蒙古马 高负荷运动 内参基因 MSTN CKM |
Analysis of the Expression of MSTN Gene and CKM Gene before and after High Load Exercise Training in Mongolian Horses |
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| Affiliation: | ,College of Animal Science, Inner Mongolia Agricultural University, Inner Mongolia Mongolian Horse Genetic Resources Protection and Industrial Engineering Laboratory |
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| Abstract: | In order to investigate the expression level of MSTN and CKM gene, before and after high load exercise Training in Mongolian horse. We estimated the expression stability in skeletal muscle of 4 commonly used reference genes(TTN, 18 s r RNA GAPDH, β-actin) with SYBR Green ? quantitative RT-PCR. After determined reference gene, we measured transcriptional levels of MSTN gene and CKM gene. The results showed that the expression stability values in skeletal muscle of Mongolian horses were GAPDH(1.087)> β-actin(1.211)> TTN(1.285)> 18 s r RNA(1.460)In turn, and the GAPDH gene was selected as the reference gene for its highest stability in this experiment. After high load exercise training, the m RNA expression of MSTN gene increased than training before, and CKM gene were decreased. This study laid the foundation for the gene research related to racing performance in Mongolian horses. |
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| Keywords: | Mongolian horse High load exercise Reference gene MSTN CKM |
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