PCR扩增TK基因检测鸡传染性喉气管炎病毒的研究

根据已发表序列设计一对包含鸡传染性喉气管炎病毒（ILTV）TK基因全长核苷酸的1259bp引物，对2株ILTV强毒和1株ILTV疫苗毒进行PCR扩增，均能扩增出预期大小的目的片段，酶切分析证实了目的片段的特异性，而其它禽病原体的扩增均为阴性。PCR检测ILTV DNA的最小检测量为75pg。此方法检测人工接种鸡的气管棉拭样品，均能检测到ILTV，因此可用于临诊样品鸡传染性喉气管炎病的检测和诊断。

Detection of infectious laryng otracheitis virus with TK gene by pdymerase chain reaction

A pair of primers which inculded infectious laryngotracheitis virus (ILTV) TK gene was desiged and synthesized.When PCR was performed with 2 virulent ILTV , 1 vaccine ILTV and other pathogenic microorganisms,the amplified fragment of ILTV was about 1 259 bp which could be confirmed by restriction endonuclease analysis.,others were negative.75pg ILTV DNA could be detected by PCR. All of the tracheal sponge samples from experimentally infected chickens were amplified a specific 1 259 bp fragment of TK gene. The results suggested that the method can be used for detection and diagnosis of ILTV in clinic samples.