| 玉米骨干自交系品质基因SSR标记遗传多样性研究 |
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| 引用本文： | 铁双贵,张莉,卫敏,孙建军,卢彩霞. 玉米骨干自交系品质基因SSR标记遗传多样性研究[J]. 河南农业科学, 2005, 0(11): 27-29 |
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| 作者姓名： | 铁双贵 张莉 卫敏 孙建军 卢彩霞 |
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| 作者单位： | 1. 河南省农业科学院粮食作物研究所,河南,郑州,450002
2. 济源市农业科学研究所,河南,济源,454652 |
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| 基金项目： | 河南省杰出人才创新基金(0221001000)，国家“863”分子育种重大专项课题(2003AA207070) |
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| 摘 要： | 选用玉米品质基因第2条染色体上的2对和第7条染色体上的3对SSR引物,对32个玉米自交系材料进行遗传多样性分析。结果表明:不同引物在不同自交系间多样性范围与程度都不相同,umc1066具有最好的多态性,带型稳定,重复性好。在o2基因内的SSR标记区域变异具有独立性,并非保守序列。具有o2基因的自交系与不具有o2基因的自交系,在基因的等位区段具有明显差异;在都具有o2基因的自交系间o2基因区段内的SSR标记具有多样性;具有o2基因的材料与优良自交系之间具有较好的多态性。
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| 关 键 词： | 玉米 SSR标记 遗传多样性 |
| 文章编号： | 1004-3268(2005)11-0027-03 |
| 收稿时间： | 2005-08-03 |
| 修稿时间： | 2005-08-03 |
Studies on Genetic Diversity of Quality Gene in Inbred Lines of Maize Based on SSR Markers |
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| TIE Shuang-gui,ZHANG Li,WEI Min,SUN Jian-jun,LU Cai-xia. Studies on Genetic Diversity of Quality Gene in Inbred Lines of Maize Based on SSR Markers[J]. Journal of Henan Agricultural Sciences, 2005, 0(11): 27-29 |
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| Authors: | TIE Shuang-gui ZHANG Li WEI Min SUN Jian-jun LU Cai-xia |
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| Affiliation: | 1. The Cereal Crops Insititute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2. Agricultural Institute of Jiyuan City, Jiyuan 454652, China |
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| Abstract: | A set of 2 maize SSR primers located on the 2th maize chromosomes and a set of 3 maize SSR primers located on the 7th maize chromosomes were used to investigate the genetic diversity of 32 inbred lines of maize.The result showed that all SSR primers were distinct in the range and degree of diversity in different inbred lines.Umc1066 had the best diversity.The SSR markers areas in o2 gene had independent character in variation and they weren't conservative sequence.There was a good diversity in between the materials with o2 gene and good inbred lines.Which layed the good foundation for setting up the SSR marker-assisted breeding system of QPM.SSR markers play an important role in variety identification of inbred lines of maize and study of genetic diversity. |
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| Keywords: | Maize SSR markers Genetic diversity |
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